This Gamma-irradiated product retains inflammatory activity. The minimum potency of this product is 500000 EU/mg. Irradiation can partially alter polysaccharide regions, while lipid A — the main driver of TLR4 activation — remains relatively less affected. Therefore, inflammatory signaling may persist but with reduced potency as compared to non-irradiated LPS. Please see the link below to review a sample or lot-specific Certificate of Analysis, which includes potency:
https://www.sigmaaldrich.com/product/sigma/l4516#product-documentation
Fortfahren mit
L4516
Lipopolysaccharide aus Escherichia coli O127:B8
suitable for cell culture, BioXtra, γ-irradiated
Synonym(e):
LPS
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 mg | Warenkorb auf Verfügbarkeit prüfen | € 94,90 |
Über diesen Artikel
Technischer Dienst
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Unterstützung erhaltenbiological source
Escherichia coli (O127:B8)
Quality Segment
sterility
γ-irradiated
product line
BioXtra
form
lyophilized powder
purified by
gel-filtration chromatography
technique(s)
cell culture | mammalian: suitable
impurities
<1% Protein (Lowry)
solubility
H2O: 5 mg/mL, slightly hazy
storage temp.
2-8°C
Application
Lipopolysaccharide aus Escherichia coli 0127:B8 werden für folgende Anwendungen verwendet:
- zur Untersuchung des LPS-induzierten Überlebens von Makrophagen in Mäusen
- als Auslöser der Zytokinsekretion durch Rinder-PBMC
- zur Untersuchung der Apoptose hochempfindlicher Makrophagenzellen in einer LPS-induzierten proinflammatorischen Umgebung
- zur Ausführung eines Autophagie-Assays in BV2- und BV2-LC3-Zellen
- zur Stimulation von menschlichen Monozyten und Makrophagen sowie von Mikrogliazellen in Mäusen
- zur Induktion der Expression von Pentraxin-3 in 293T-Zellen
Lipopolysaccharide (LPS) sind charakteristische Bestandteile der Zellwand von gramnegativen Bakterien. LPS und seine Lipid-A-Gruppe stimulieren Zellen des angeborenen Immunsystems durch den Toll-like-Rezeptor 4 (TLR4). TLR4 gehört zur Toll-like-Rezeptor-Proteinfamilie, die häufige Pathogen-assoziierte molekulare Strukturen (PAMPs) erkennt.
Biochem/physiol Actions
Preparation Note
Lipopolysaccharide werden als lyophilisierte, γ-bestrahlte Pulver geliefert. Zur Rekonstitution dem Fläschchen (1 mg) 1 mL einer ausgeglichenen, sterilen Kochsalzlösung oder eines Zellkulturmediums zugeben und behutsam schwenken, bis sich das Pulver auflöst. Das rekonstituierte Produkt kann mit einer ausgeglichenen, sterilen Kochsalzlösung oder einem Gewebekulturmedium weiter bis auf die erwünschten Arbeitskonzentrationen verdünnt werden.
Other Notes
Für einen umfassenden Überblick über unser vielfältiges Angebot an Lipopolysacchariden für Ihre Forschung empfehlen wir Ihnen den Besuch unserer Seite zur Kategorie Kohlenhydrate.
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Dieser Artikel | |||
|---|---|---|---|
| description suitable for cell culture, BioXtra, γ-irradiated | description Ready Made solution, 1 mg/mL | description purified by phenol extraction | description purified by gel-filtration chromatography |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) - |
| biological source Escherichia coli (O127:B8) | biological source Escherichia coli (O127:B8) | biological source Escherichia coli (O127:B8) | biological source Escherichia coli (O127:B8) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| solubility H2O: 5 mg/mL, slightly hazy | solubility water: soluble | solubility water: soluble | solubility water: soluble |
| form lyophilized powder | form buffered aqueous solution | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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Signalwort
Danger
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Acute Tox. 2 Oral
Lagerklasse
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
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Does the γ-irradiation of the powder break the structure of LPS and consequently alter its ability to trigger inflammatory activation?
1 answer-
Helpful?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Once reconstituted what is the stability at -20C for the LPS product L4516-1M?
1 answer-
Frozen aliquots can be stored up to 2 years. For more information, please see the link below:
https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharidesHelpful?
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Can LPS bind to plastics (ex. 1.5 mL microtube) even when LPS solution is frozen stored at -20℃? I store LPS solution at 0.05mg/mL in -20℃ now.
1 answer-
LSP is less likely to stick to plastics than glass, however very low concentrations (<0.1 mg/mL in water), LPS tends to stick to containers made
of certain types of glass or plastic. To minimize, prepare stock solutions of at least 1 mg/mL. If the LPS concentration is >1 mg/mL, adsorption to the sides of the vial is
negligible. For solutions stored in glass, vortexing for 30 minutes following thaw is recommended to redissolve. This may be useful for dilute solutions in plastic, as well. Surfaces (glass and plastics) may be salinized to reduce adherence.See Sigmacote, SL2:
https://www.sigmaaldrich.com/product/sigma/sl2For additional information, see the link below to review the Lipopolysaccharides product information page:
https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharidesHelpful?
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