This product is not tested for solubility. For activity testing purposes, it is solubilized at 500 - 1000 units per mL in cold deionized water.
A similar product, L3294, Lipase from Thermus flavus, may be dissolved in 50 mM Tris-HCl, pH 8.0.
Note that this may yield a slightly hazy solution. The solution is expected to be stable for at least one month at 2–8 °C. This information may also be applied to L8525, Lipase from Candida rugosa.
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L8525
Lipase aus Candida rugosa
lyophilized powder, ≥40,000 units/mg protein
Synonym(e):
Lipase AY 30 Amano, Triacylglycerol-acylhydrolase, Triacylglycerol-lipase
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1000000 units | Warenkorb auf Verfügbarkeit prüfen | € 219,00 |
5000000 units | Warenkorb auf Verfügbarkeit prüfen | € 540,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-619-9
EG-Nummer:
MDL number:
Specific activity:
≥40,000 units/mg protein
Biological source:
fungus (Candida rugosa)
Technischer Dienst
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Unterstützung erhaltenbiological source
fungus (Candida rugosa)
Quality Segment
form
lyophilized powder
specific activity
≥40,000 units/mg protein
composition
Protein, ≥4.0%
foreign activity
α-amylase ≤0.05 unit/mg solid, protease ≤0.001 units/mg solid
storage temp.
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
General description
Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.
Application
Lipase from C. rugosa is used to catalyze the synthesis of flavor esters such as pentyl propanoate and butyl butanoate used in food enhancement.
Lipase from Candida rugosa has been used:
- in the hydrolysis of loaded polycaprolactone–20% tricalcium phosphate (PCL–TCP) composites
- in adsorption of proteins in emulsan/alginate beads and in enzyme assays
- to catalyse methanolysis and hydrolysis reactions
Lipase from Candida rugosa has been used in a study to assess the influence of phase segregation on enzyme hydrolytic activity.
Lipasen werden in der Industrie zum Auflösen von chiralen Verbindungen und zur Umesterung bei der Herstellung von Biodiesel verwendet.
Biochem/physiol Actions
Lipases are used in detergent, food, leather, pharmaceutical and diary industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.
Tri-, Di- und Monoglyceride werden hydrolysiert (in abnehmender Reihenfolge der Rate).
Tri-, Di- und Monoglyceride werden hydrolysiert (in abnehmender Reihenfolge der Rate).
Lipasen katalysieren die Hydrolyse von Triacylglycerinen zu Glycerin und freien Fettsäuren.
Lipasen katalysieren die Hydrolyse von Triacylglycerinen zu Glycerin und freien Fettsäuren.
Analysis Note
Protein determined by biuret.
Other Notes
One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37 °C using olive oil (30 minute incubation).
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description lyophilized powder, ≥40,000 units/mg protein | description Type VII, ≥700 unit/mg solid | description powder, yellow-brown, ≥2 U/mg | description lyophilized, powder (fine), 15-25 U/mg |
| biological source fungus (Candida rugosa) | biological source (from Candida rugosa) | biological source (from Candida rugosa) | biological source fungus (Candida rugosa) |
| specific activity ≥40,000 units/mg protein | specific activity ≥700 unit/mg solid | specific activity ≥2 U/mg | specific activity 15-25 U/mg |
| form lyophilized powder | form lyophilized | form powder | form powder (fine), lyophilized solid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| foreign activity α-amylase ≤0.05 unit/mg solid, protease ≤0.001 units/mg solid | foreign activity - | foreign activity - | foreign activity - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
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Signalwort
Danger
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, type N95 (US)
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