product M1255, Calcein Blue to is used to stain cells, but it will stain living or dead cells. For a cell viability stain, one can use Calcein Blue AM. The difference between Calcein Blue and Calcein Blue AM is the methyl-iminodiaceitc acid group that chelates the divalent metal has acetoxymethyl esters on it in Calcein Blue AM. The esters are cleaved inside of viable cells, but remain on the molecule in the absence of active esterases. So, if you use M1255 it will stain all the cell whether alive or dead.
Fortfahren mit
M1255
Calceinblau
Powder
Synonym(e):
7-Hydroxy-4-methyl-8-cumarinylmethyliminodiessigsäure
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 g | Warenkorb auf Verfügbarkeit prüfen | € 97,80 |
10 g | Warenkorb auf Verfügbarkeit prüfen | € 472,00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C15H15NO7
CAS-Nummer:
Molekulargewicht:
321.28
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
259-129-8
MDL number:
Technischer Dienst
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Unterstützung erhaltenProduktname
Calceinblau, fluorescent dye
Quality Segment
form
powder
technique(s)
titration: suitable
color
white to light yellow
solubility
1 M NaOH: 50 mg/mL
fluorescence
λex 322 nm; λem 445 nm in methanol
suitability
suitable for indicator for EDTA titration
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12
InChI
1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)
InChI key
RJPSHDMGSVVHFA-UHFFFAOYSA-N
General description
Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.
Application
Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.
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Dieser Artikel | |||
|---|---|---|---|
| description fluorescent dye | description Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium. | description suitable for fluorescence, BioReagent, ≥90% (HPLC) | description 4 mM in DMSO, ≥90% (HPLC), solution |
| technique(s) titration: suitable | technique(s) titration: suitable | technique(s) - | technique(s) titration: suitable |
| suitability suitable for indicator for EDTA titration | suitability suitable for (Suitable for fluorometric determination of Ca Suitable for EDTA titration of Ca in the presence of Mg) | suitability suitable for fluorescence | suitability - |
| color white to light yellow | color yellow to brown | color - | color colorless, white to off-white |
| fluorescence λex 322 nm; λem 445 nm in methanol | fluorescence λex 470 nm; λem 509 nm (pH 7.4) | fluorescence λex 496 nm; λem 516 nm in 0.1 M Tris pH 8.0, esterase; Ca2+ | fluorescence - |
| solubility 1 M NaOH: 50 mg/mL | solubility NaOH: 50 mg/mL | solubility - | solubility - |
| form powder | form powder | form solid | form solution |
Signalwort
Warning
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Zielorgane
Respiratory system
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 3
PSA (Persönliche Schutzausrüstung)
dust mask type N95 (US), Eyeshields, Gloves
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Is Calcein Blue identical to Calcein Blue AM, and if not, what is the difference? Would it be compatible in a flourescent microscopy assay looking at %viability of THP-1 cells?
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