2-Mercaptoethanol is compatible with a wide range of common aqueous buffers, such as Laemmli sample buffer, phosphate-buffered saline, or Tris buffer. Certain buffers, such as Tris, can cause oxidation of 2-mercaptoethanol. In these situations, preparation of the solution is recommended just before use.
M3148
2-Mercaptoethanol
Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
Synonym(e):
β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylenglycol
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Über diesen Artikel
Lineare Formel:
HSCH2CH2OH
CAS-Nummer:
Molekulargewicht:
78.13
Beilstein:
773648
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12161504
PubChem Substanz-ID:
NACRES:
NA.31
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Unterstützung erhaltenQualität
Molecular Biology
Dampfdichte
2.69 (vs air)
Dampfdruck
1 mmHg ( 20 °C)
Produktlinie
BioReagent
Assay
99% (GC/titration)
Form
liquid
Expl.-Gr.
18 %
Eignung der Reaktion
reagent type: reductant
Konzentration
14.3 M (pure liquid)
Methode(n)
cell culture | mammalian: suitable
electrophoresis: suitable
Brechungsindex
n20/D 1.500 (lit.)
pH-Wert
4.5-6 (20 °C, 500 g/L)
bp
157 °C (lit.)
Löslichkeit
H2O: soluble 1 mL/mL
Dichte
1.114 g/mL at 25 °C (lit.)
Fremdaktivität
DNase, RNase, protease, none detected
Lagertemp.
2-8°C
SMILES String
OCCS
InChI
1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
InChIKey
DGVVWUTYPXICAM-UHFFFAOYSA-N
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Dieser Artikel | 63689 | 8.05740 | M6250 |
|---|---|---|---|
| grade for molecular biology | grade for molecular biology | grade - | grade - |
| technique(s) cell culture | mammalian: suitable, electrophoresis: suitable | technique(s) RNA extraction: suitable, protein extraction: suitable | technique(s) - | technique(s) - |
| assay 99% (GC/titration) | assay ≥99.0% (GC) | assay ≥99% (GC) | assay ≥99.0% |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| product line BioReagent | product line BioUltra | product line - | product line - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-30°C | storage temp. 2-8°C |
Anwendung
BME ist geeignet für die Reduzierung von Protein-Disulfidbindungen vor der Polyacrylamidgel-Elektrophorese und ist für gewöhnlich in einer Konzentration von 5 % in einem Probenpuffer für SDS-PAGE enthalten. Die Aufspaltung intermolekularer Disulfidbindungen (zwischen Untereinheiten) ermöglicht, dass sich die Untereinheiten eines Proteins bei der SDS-PAGE eigenständig voneinander trennen. Die Aufspaltung intramolekularer Disulfidbindungen (innerhalb von Untereinheiten) sorgt dafür, dass die Untereinheiten komplett denaturiert werden, sodass die einzelnen Peptide gemäß ihrer Kettenlänge und nicht in Abhängigkeit ihrer Sekundärstruktur migrieren.
Reduktionsmittel
β-Mercaptoethanol oder 2-Mercaptoethanol wurde verwendet:
β-Mercaptoethanol oder 2-Mercaptoethanol wurde verwendet:
Signalwort
Danger
Gefahreneinstufungen
Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
Zielorgane
Liver,Heart
Lagerklassenschlüssel
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flammpunkt (°F)
154.4 °F - closed cup
Flammpunkt (°C)
68 °C - closed cup
Persönliche Schutzausrüstung
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Protokolle
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In which buffer or solvent should 2-Mercaptoethanol be diluted for working solution preparation?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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I’ve purchased 25mL bottle and want to use this in my cell culture media at concentration of 50mM. What is the concentration in the 25mL bottle and how to make this to 50mM concentration?
1 answer-
This product is a neat or pure material. The concentration is considered to be 100%. The calculated molarity is 14.3 M as reported in the Properties section of the product page.
To prepare a 50 mM solution, please mix 0.391 g of M3148 with 100 mL solvent. 0.391 g / 78.13 g/mol / 0.1 L = 0.05 = 50 mM.Helpful?
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Hi, What is the concentration of 2-mercaptoethanol in the 100mL bottle?
1 answer-
This product is a neat or pure material. The concentration is considered to be 100%. The calculated molarity is 14.3 M as reported in the Properties section of the product page.
Helpful?
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Hi is this sterile?
1 answer-
This product is not sterile, but can be used in cell culture applications and is DNase, RNase, and protease free.
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