M7522

2-Mercaptoethanol

liquid, BioReagent, suitable for cell culture

• Synonym(e): β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylenglycol
• Lineare Formel: HSCH₂CH₂OH
• CAS-Nummer: 60-24-2
• Molekulargewicht: 78.13
• EC Number: 200-464-6
• UNSPSC Code: 12352200
• PubChem Substance ID: 24897168
• Beilstein/REAXYS Number: 773648
• MDL number: MFCD00004890

Eigenschaften

• vapor density: 2.69 (vs air)
• vapor pressure: 1 mmHg ( 20 °C)
• product line: BioReagent
• form: liquid
• expl. lim.: 18 %
• concentration: 14.3 M (pure liquid)
• technique(s): cell culture | mammalian: suitable
• refractive index: n20/D 1.500 (lit.)
• bp: 157 °C (lit.)
• solubility: H₂O: 1 mL/mL
• density: 1.114 g/mL at 25 °C (lit.)
• SMILES string: OCCS
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI key: DGVVWUTYPXICAM-UHFFFAOYSA-N

Beschreibung

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

This product has been replaced by M3148-SIGMA | 2-Mercaptoethanol for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Sicherheitshinweise

• Piktogramme: GHS06,GHS08,GHS05,GHS09
• Signalwort: Danger
• Gefahrencodes: H301 + H331,H310,H315,H317,H318,H361f,H373,H410
• Sicherheitshinweise: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• Zielorgane: Liver,Heart
• Lagerklasse: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Flammpunkt (°F): 165.2 °F - closed cup
• Flammpunkt (°C): 74 °C - closed cup
• PSA (Persönliche Schutzausrüstung): Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter