The enzyme included in the kit is capable of reacting with tyrosine, methionine, and phenylalanine. According to a specific article, L-phenylalanine dehydrogenase can convert tyrosine and methionine in addition to phenylalanine.
It's important to note that the enzyme is isolated from bacteria, not humans. The reaction pathway, likely catalyzed by phenylalanine dehydrogenase, is as follows: L-phenylalanine + H2O + NAD+ ⇌ phenylpyruvate + NH3 + NADH + H+.
It was mentioned that any additional information about the enzyme would be proprietary. However, it was disclosed that this proprietary enzyme aids in the conversion of phenylalanine to generate NADH. In this process, PHE is reductively deaminated with the simultaneous formation of NADH, which reacts with our fluorescent probe to produce fluorescence at Ex/Em = 535/587 nm. It is advisable to proceed with the understanding that the reaction follows the pathway outlined in my previous note.