MSKT0004

IA-MS Immunoaffinity Mass Spectrometry Kit - Protein A/G

96 Well Strip Plate with SOLu-Trypsin

• Synonym(e): Target protein enrichment and quantification from complex background by mass spectrometry.
• NACRES: NA.32
• UNSPSC Code: 41115859

Eigenschaften

• form: solid
• technique(s): protein mass spectrometry: suitable
• suitability: suitable for mass spectrometry
• shipped in: wet ice
• storage temp.: 2-8°C

Beschreibung

General description

The Protein A/G IA-MS Immunoaffinity Mass Spectrometry Kit is a 96-well platform enabling robust, high-throughput quantification of proteins in complex backgrounds by LC-MS/MS. Users provide their own capture antibody and internal standard (available separately, internal standards may be SIL peptides or preferably full length SIL protein) and the kit contains all other components needed for in-well enrichment and rapid trypsin digestion of samples. The plate format consists of 8-well strips allowing for partial runs, and avoids the high cost and potential LC column blockage associated with bead-based enrichment formats. The entire workflow can be performed in under 5 hours, resulting in digests ready for injection with no SPE or other cleanup required.

Application

Target protein enrichment and quantification from complex background by mass spectrometry.

Features and Benefits

• Robust, high-throughput quantification of proteins in complex backgrounds by LC-MS/MS
• Entire workflow including digest completed in under 5 hours, such that samples are injection ready at the end of the work day
• 96-well plate format has potential for automation in a liquid-handling system
• Bead-free system circumvents messy clean-up and eliminates risk of column clogging
• Plate strips allow user to run as many or as few samples as desired

Packaging

Box with foam insert containing kit components.

Preparation Note

2 years when stored at 2-8 °C.

Related Presentation

• Development and Comparison of Two High Throughput LC-MS Methods for the Accurate Quantitation of IGF1 in Human Serum (Poster, PDF)

Other Notes

Protein A/G Coated Plates SAE0113-1EA
SOLu-Trypsin EMS0004-100UG
MS Denaturation Solution EMS00010-10ML
Rapid Trypsin Digestion Buffer EMS0009-30ML
Tris Buffered Saline with TWEEN^® 20 (TBST)powder, pH 8.0 T9039
Tris Buffered Saline (TBS) powder, pH 8.0 T6664
EZ-Pierce^™ plate seal Z721581-1EA

See datasheet for more information.

Legal Information

EZ-Pierce is a trademark of Excel Scientific, Inc.

TWEEN is a registered trademark of Croda International PLC

Nur Kit-Komponenten

• MS Denaturation Solution
• Rapid Trypsin Digestion Buffer

Kit-Komponenten auch einzeln erhältlich

• Protein A/G Coated Plates

  SDB
• EMS0004

  SOLu-Trypsin, recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid

  SDB
• T9039

  Tris Buffered Saline, with Tween™ 20, pH 8.0, powder

  SDB
• T6664

  Tris Buffered Saline, pH 8.0, powder

  SDB
• Z721581

  EZ-Pierce^™ films, Excel Scientific EZP-100, non-sterile

  SDB

Sicherheitshinweise

• Piktogramme: GHS02,GHS06,GHS08
• Signalwort: Danger
• Gefahrencodes: H226,H301 + H311 + H331,H370
• Sicherheitshinweise: P210 - P233 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 3 - STOT SE 1
• Zielorgane: Eyes,Central nervous system
• Lagerklasse: 3 - Flammable liquids
• Flammpunkt (°F): 75.2 °F
• Flammpunkt (°C): 24 °C
• Was ist los?: WGK 3