The concentration in U/mL can be determined using the Enzymatic Activity and the mg protein/mL value reported on the lot specific Certificate of Analysis. For example, a lot has a reported Enzymatic Activity of 359 units/ mg protein and 13.7 mg protein/ mL will have a concentration 4918.3 units/ml. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/US/en/product/sigma/p1506#product-documentation
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P1506
Pyruvat-Kinase aus Kaninchenmuskel
Type II, ammonium sulfate suspension, 350-600 units/mg protein
Synonym(e):
ATP:pyruvate 2-O-phosphotransferase, PK, Phospho-enolpyruvat-Kinase
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1000 units | Warenkorb auf Verfügbarkeit prüfen | € 46,20 |
5000 units | Warenkorb auf Verfügbarkeit prüfen | € 142,00 |
25000 units | Warenkorb auf Verfügbarkeit prüfen | € 430,00 |
50000 units | Warenkorb auf Verfügbarkeit prüfen | € 713,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
EG-Nummer:
MDL number:
Specific activity:
350-600 units/mg protein
Biological source:
rabbit muscle
Technischer Dienst
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Unterstützung erhaltenbiological source
rabbit muscle
Quality Segment
type
Type II
form
ammonium sulfate suspension
specific activity
350-600 units/mg protein
mol wt
237 kDa
storage condition
(Tightly closed)
technique(s)
ligand binding assay: suitable
color
white
foreign activity
lactic dehydrogenase, creatine phosphokinase, and myokinase ≤0.01%, phosphoglucomutase ≤0.05%
storage temp.
2-8°C
General description
Application
Biochem/physiol Actions
Pyruvate kinase from rabbit muscle can be activated by histidine and inhibited by low levels of zinc (Zn2+). In the glycolytic pathway, pyruvate kinase (PK) functions as a terminal enzyme, catalyzing the conversion of phosphoenolpyruvate to pyruvate and the synthesis of ATP through substrate-level phosphorylation. Tetrameric structures of PK are more active and have a high affinity for phosphoenolpyruvate (PEP), whereas dimeric structures are less active and have a low affinity for PEP. PK from rabbit muscle possesses positive kinetic cooperativity (Hill coefficient> 1.35) of the phosphoenol pyruvate and adenosine diphosphate(ADP) binding sites.
Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path
Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path
Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.
Physical form
Suspension in 3.2 M (NH4)2SO4 solution, pH 6
Analysis Note
Protein determined by biuret.
Other Notes
One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37 °C.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description Type II, ammonium sulfate suspension, 350-600 units/mg protein | description Type III, lyophilized powder, 350-600 units/mg protein | description Type VII, buffered aqueous glycerol solution, 350-600 units/mg protein | description from rabbit muscle |
| biological source rabbit muscle | biological source rabbit muscle | biological source - | biological source rabbit muscle |
| specific activity 350-600 units/mg protein | specific activity 350-600 units/mg protein | specific activity 350-600 units/mg protein | specific activity ~200 units/mg protein (At 25 °C with PEP as the substrate.) |
| technique(s) ligand binding assay: suitable | technique(s) - | technique(s) - | technique(s) - |
| form ammonium sulfate suspension | form lyophilized powder | form buffered aqueous glycerol solution | form solution |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C (−15°C to −25°C) |
| storage condition (Tightly closed) | storage condition - | storage condition - | storage condition - |
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Lagerklasse
12 - Non Combustible Liquids
Was ist los?
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Zulassungslistungen
Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.
7783-20-2
CAS No.
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この製品(P1506, ピルビン酸キナーゼ)についてご質問があります。 この製品の比活性は350-600 units/mg proteinと記載ありますが、濃度としてU/mLはお分かりになるのでしょうか。 Lot毎に異なることかもしれませんので、試薬の瓶そのものに記載があったりするのでしょうか(データシートには記載が見当たらない)。 ご確認のほど、よろしくお願いいたします。
1 answer-
Helpful?
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Hi, How long can this be stored in the fridge?
1 answer-
This product is not assigned an expiration date or retest date. Products that are robust in nature and extremely stable, in the original unopened container, are not assigned an expiration date or retest schedule. These products will have no dating reported on either the label or the Certificate of Analysis. Products that are not assigned an expiration or retest date are covered by a warranty period of 1 year from the date of product shipment.
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