Protein A–Agarose

Name: Protein A–Agarose
Brand: SIGMA

saline suspension

Synonym(e):

Protein A resin

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Über diesen Artikel

UNSPSC Code:

41106500

NACRES:

NA.56

MDL number:

MFCD00165563

Form:

saline suspension

Biological source:

Staphylococcus aureus

Technischer Dienst

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Unterstützung erhalten

Eigenschaften

biological source

Staphylococcus aureus

Quality Segment

200

form

saline suspension

extent of labeling

~2 mg per mL

technique(s)

antibody protein purification: suitable

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥15 mg/mL binding capacity (human IgG)

storage temp.

2-8°C

Beschreibung

General description

Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.

Application

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Protein A−Agarose has been used in the immunoprecipitation

of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
of prolyl hydroxylase domain 2 N27 cells
of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells

Biochem/physiol Actions

Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications.