P4300

Pektinase aus Rhizopus sp.

suitable for plant cell culture, BioReagent, crude powder

• Synonym(e): Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase
• CAS-Nummer: 9032-75-1
• UNSPSC Code: 12352200
• EC Number: 232-885-6
• MDL number: MFCD00131809
• EG-Nummer: 3.2.1.15 (BRENDA, IUBMB)

Eigenschaften

• product line: BioReagent
• form: crude powder
• specific activity: 400-800 units/g solid
• technique(s): cell culture | plant: suitable
• storage temp.: −20°C

Beschreibung

General description

Pectinase P4300 is a natural pectinase (polygalacturonase) derived from fungus Rhizops sp. that randomly hydrolyzes the (1→4)-α-D-galactosiduronic linkages in pectate and other galacturonans. This enzyme breaks down pectin found in the middle lamella of plant cell walls. Pectinase helps facilitate the maceration of plant materials and extraction processes. Pectinase P4300 is used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Application

Zur Herstellung von Pflanzenprotoplast für den Zellwandabbau vor der organellen Isolation.

Other Notes

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)