Mixing the two phenol layers does not damage this product. Phase separation can occur during handling. If layers mix, allow the solution to stand until a clear phase separation is re-established before pipetting. Temporary haziness or a visible interface after mixing is normal. Pipetting should occur only after full separation to avoid aqueous carryover and ensure consistent performance. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/154/087/p4557dat.pdf
Fortfahren mit
P4557
Phenol -Lösung
BioReagent, Molecular Biology
Synonym(e):
Tris-equilabrated Phenol
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 mL | Warenkorb auf Verfügbarkeit prüfen | € 83,30 |
400 mL | Warenkorb auf Verfügbarkeit prüfen | € 313,00 |
Über diesen Artikel
Lineare Formel:
C6H5OH
CAS-Nummer:
Molekulargewicht:
94.11
UNSPSC Code:
12352104
NACRES:
NA.52
PubChem Substance ID:
EC Number:
203-632-7
Beilstein/REAXYS Number:
6428463
MDL number:
Assay:
≥99.0% (GC)
Grade:
Molecular Biology
€ 83,30
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltengrade
Molecular Biology
Quality Segment
product line
BioReagent
assay
≥99.0% (GC)
form
liquid
dilution
(for analytical testing)
pH
7.7-8.1 (25 °C)
storage temp.
2-8°C
SMILES string
Oc1ccccc1
InChI
1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
InChI key
ISWSIDIOOBJBQZ-UHFFFAOYSA-N
Gene Information
human ... GABRA1(2554)
General description
Eine Tris-äquilibrierte Phenollösung ist eine klare Flüssigkeit, die mit einer Tris-Pufferschicht abgedeckt ist. Phenollösungen können in zahlreichen molekularbiologischen Anwendungen zum Einsatz kommen. Organische Verbindungen wie Phenole besitzen einen aromatischen carbocyclischen Ring, an den direkt eine Hydroxylgruppe gebunden ist. Der Trivialname für Monohydroxybenzol ist Phenol.
Application
Phenollösung wird verwendet:
- als Bestandteil der Aufschlussreaktionsmischung zum Herstellen von RNase-freien Plasmiden für die In-vitro-Translation in Kaninchen-Retikulozytenlysat
- zur Extraktion von DNA aus Rosettenblättern von Arabidopsis thaliana
- zur Extraktion von DNA aus Blut auf Konservierungspapier mit einem gängigen Phenol-Chloroform-Protokoll
- als Komponente von Extraktionspuffer zum Extrahieren von RNA aus Fruchtfleisch
- zur Extraktion von Protein aus Wurzeln
- zur Isolierung von fäkaler DNA
- zur Extraktion von Caged-RNA
Biochem/physiol Actions
Die Phenol-Chloroform-Extraktionsmethode wird häufig zur Extraktion von Nukleinsäuren eingesetzt. Auch wenn Phenol die RNase-Aktivität nicht vollständig hemmt, ist es aufgrund seiner einzigartigen Eigenschaften in Kombination mit Chloroform und Alkohol für die Isolierung von Nukleinsäuren nützlich.
Other Notes
- 1 Flasche Tris-äquilibriertes Phenol (Produktnummer P4557)
- Equilibrierungspuffer (Produktnummer B5658)
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description BioReagent, Molecular Biology | description certified reference material, 500 μg/mL in methanol | description 5000 μg/mL in methanol, certified reference material | description Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, BioReagent, Molecular Biology |
| grade Molecular Biology | grade certified reference material, TraceCERT® | grade certified reference material, TraceCERT® | grade Molecular Biology |
| Quality Level 200 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| assay ≥99.0% (GC) | assay - | assay - | assay - |
| form liquid | form - | form - | form liquid |
| pH 7.7-8.1 (25 °C) | pH - | pH - | pH 4.1-4.5 (25 °C) |
| product line BioReagent | product line TraceCERT® | product line TraceCERT® | product line BioReagent |
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Signalwort
Danger
Gefahrencodes
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
Zielorgane
Nervous system,Kidney,Liver,Skin
Lagerklasse
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Was ist los?
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
Hier finden Sie alle aktuellen Versionen:
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
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What happens if I mix the two layers of phenol before pipetting? I prepared FIAC this way
1 answer-
Helpful?
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How long can you store phenol for, after adding the buffer?
1 answer-
Following the addition of buffer, the solution may be stored for 6 months at 2–8 °C. For extended storage after opening, with or without the addition of buffer, the material may be stored at –20 °C, protected from light. This information is available in the product datasheet. Please see the link below to review additional details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/154/087/p4557dat.pdfHelpful?
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Esta botella de Fenol (10 mM TrisHCl pH 8.0) viene junto con una botella pequeña de tampón Tris a pH 10.5 for saturated phenol. Se supone que debo añadírsela a la botella de fenol para ajustar a 10 mM TrisHCl?
1 answer-
Yes, this phenol solution includes a separate bottle of equilibration buffer (Product No. B5658) to adjust the phenol phase to pH 7.9±0.2. The entire contents of the bottle of the buffer should be added to the phenol.
Helpful?
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What is the export packaging? Is it in thermocol/Foam packaging? Can you share the packaging Image.
1 answer-
Both package sizes are bottles that are sealed in a polyamide bag. This bag is placed, with additional absorbent material and padding, in a metal can with a pull tap. The final packaged item is placed in the shipping box that is most compatible with the order. This product is shipped at ambient temperature.
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What is the final pH of Phenol solution, Product P4557, after adding the buffer?
1 answer-
The pH is typically 8.0; the specifications state a range of 7.7-8.1.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What do I do to equilibrate the Phenol solution, Product P4557, to around pH 8?
1 answer-
This phenol solution (Product No. P4557) includes a separate bottle of equilibration buffer (Product No. B5658) to adjust the phenol phase to pH 7.9±0.2. After addition of the buffer to the phenol, it may take about 6 hours (mixture can be kept at 2-8 °C) until the bottom phase (phenol) becomes clear. Some of the haziness in the phenol phase may be due to some air bubbles caused by mixing by inversion. There could be a hazy interface between the phenol and aqueous phase which remains after the separation. The entire contents of the bottle of the buffer should be added to the phenol. If buffering less than the complete bottle, use at a ratio of 6.5 mL buffer to 100 mL phenol. For 10 mL of phenol, use 650 μL of buffer. For 1 mL of phenol, use 65 μL of buffer.
Helpful?
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What is the molarity of Phenol solution, Product P4557?
1 answer-
The Molarity is ~10.6. The density of the phenol is 1.05g/ml, and the molecular weight is 94.11.
Helpful?
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I see that there are two layers in the bottle Phenol solution, Product P4557. Which layer do I use?
1 answer-
The top phase is Tris buffer, so use the lower/bottom phase.
Helpful?
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