PLX15
GenElute™ Plasmid Maxiprep Kit
sufficient for 15 purifications
Nachhaltigkeitsmerkmale
Synonym(e):
Gen Elute, GenElute™ Plasmid Kit
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NACRES:
NA.52
UNSPSC Code:
41105501
Technischer Dienst
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Unterstützung erhaltenusage
sufficient for 15 purifications
Quality Segment
sustainability
Sustainability features
technique(s)
DNA purification: suitable
test parameters
: ~45 min kit run time, sample volume: 25-200 mL bacterial culture
storage temp.
15-25°C
General description
The GenElute Plasmid Maxiprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 1.2 mg of plasmid DNA can be recovered from 25-200 ml of Luria Broth (LB) in about 45 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
This product aligns with one or more of the 12 Principles of Green ChemistryThis product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
This product aligns with one or more of the 12 Principles of Green ChemistryThis product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
The recovered plasmid DNA is predominantly in its supercoiled form. There is no visual evidence of genomic DNA or RNA contamination detected by agarose gel electrophoresis. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, and transfection.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Signalwort
Danger
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Zielorgane
Central nervous system
Lagerklasse
10 - Combustible liquids
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
WGK
WGK 3
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