RAB0036

Human Carbonic Anhydrase IX ELISA Kit

for serum, plasma, cell culture supernatant and urine

• Synonym(e): CA9
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... CA9(768)
• Input: sample type plasma; sample type serum; sample type cell culture supernatant(s); sample type urine
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: wet ice

Eigenschaften

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type plasma; sample type serum; sample type cell culture supernatant(s); sample type urine
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 0.2 pg/mL; standard curve range: 0.205-50 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CA9(768)

Beschreibung

General description

Carbonic anhydrase IX (CA9) belongs to the zinc-containing metalloenzymes family. The protein is encoded by the gene mapped to human chromosome 9p. The encoded protein is localized on cell membrane and is characterized with an N-terminal proteoglycan-like domain (PG), a CA catalytic domain (CA), a transmembrane helical segment and a short intra-cytoplasmic tail. CA9 is expressed at very low levels in normal tissues (mostly in gastrointestinal tract), but it is expressed at high level in various cancer tissues, such as renal, lung, cervical, ovarian, esophageal and breast cancer.

The Human CA-IX (Carbonic Anhydrase IX) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CA-IX in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human CA9

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Carbonic anhydrase IX (CA9) aids in reversible hydration of carbon dioxide to carbonic acid and bicarbonate. CA9 functions as a promising biomarker for diagnosing clear cell renal cell carcinoma (ccRCC), tumor hypoxia and prostate cancer (PC). In addition to pH control, CA9 also plays a vital role in cell proliferation, adhesion and tumor invasion.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

• RABELADB

  ELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  SDB
• RABELADC

  ELISA 1X Assay/Sample Diluent Buffer C (Item L)

  SDB
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDB
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDB
• RABWASH4

  20X Wash Buffer (Item B)

  SDB

Sicherheitshinweise

• Piktogramme: GHS05
• Signalwort: Warning
• Gefahrencodes: H290
• Sicherheitshinweise: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Lagerklasse: 10 - Combustible liquids