RAB0149

Human EGF ELISA Kit

for serum, plasma, cell culture supernatant.

• Synonym(e): EGF ELISA Kit
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... EGF(1950)
• Input: sample type cell culture supernatant(s); sample type plasma; sample type serum
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: wet ice

Eigenschaften

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type cell culture supernatant(s); sample type plasma; sample type serum
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 1 pg/mL; standard curve range: 0.82-200 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... EGF(1950)

Beschreibung

General description

The Human EGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human EGF in serum, plasma and cell culture supernatants.

Immunogen

Recombinant Human EGF

Application

Human EGF ELISA Kit has been used to examine the concentration of epidermal growth factor (EGF) in adipose derived stem cell conditioned medium (ADSC-CM).

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Epidermal growth factor (EGF) is the member of EGF-like family of growth factors, which binds to EGF receptor. EGF increases proliferation and migration in bone marrow stromal cells (BMSC), that gives rise to osteoblasts, chondrocytes and adipocytes. It blocks gastric acid and pepsin secretion and enhances gastrointestinal cell proliferation. It functions as a strong mitogenic factor for various cultured cells. Mutations in this gene is associated with hypomagnesemia..

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

• RABELADA

  ELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  SDB
• RABELADB

  ELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  SDB
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDB
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDB
• RABWASH4

  20X Wash Buffer (Item B)

  SDB

Sicherheitshinweise

• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 10 - Combustible liquids
• Piktogramme: GHS08
• Signalwort: Danger