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RAB0201

GLP-1 EIA Kit

for serum, plasma, culture supernatant and cell lysates

Synonym(e):

Glucagon-like-peptide 1

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Größe/SKUVerfügbarkeitPreis
1 kit
Versand in der Regel in 1 Woche. (Bei Bestellungen außerhalb der USA und Europas rechnen Sie bitte zusätzlich 1-2 Wochen für die Lieferung ein)
€ 510,00

Über diesen Artikel

NACRES:
NA.32
UNSPSC Code:
12352200

€ 510,00


Versand in der Regel in 1 Woche. (Bei Bestellungen außerhalb der USA und Europas rechnen Sie bitte zusätzlich 1-2 Wochen für die Lieferung ein)

Technischer Dienst
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Unterstützung erhalten


species reactivity

human, rat, mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

input

sample type cell lysate
sample type plasma
sample type serum
sample type culture supernatant(s)

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.17 pg/mL
standard curve range: 0.1–1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GCG(2641)

General description

The GLP-1 Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting GLP-1 peptide based on the principle of Competitive Enzyme Immunoassay.

Immunogen

Glucagon-like Peptide 1 (GLP-1), synthetic peptide

Application

For research use only. Not for use in diagnostic procedures.

Biochem/physiol Actions

Glucagon-Like Peptide I (GLP1) controls the metabolism of glucose by inducing the secretion of insulin and suppressing that of glucagon. Studies in Indonesian population show that reduced levels of this protein are linked with increased susceptibility to type 2 diabetes mellitus. Plasma levels of this hormone also show significant association with systolic and diastolic blood pressure levels.


Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB & Preisangaben

Piktogramme

Corrosion

Signalwort

Warning

Gefahrencodes

Sicherheitshinweise

Hazard Classifications

Met. Corr. 1

Lagerklasse

10 - Combustible liquids



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Questions

1–2 of 2 Questions  
  1. Which forms of GLP-1 are measured by the GLP ELISA (RAB0201)? Is it specific to the active form 7-36aa?

    1 answer
    1. The kit RAB0201 is specific for amino acids 108-128 (P01275). This includes the active forms 7-36a and 7-37, as well as the inactive cleaved form 9-36a.

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  2. Hi, 1 unit GLP-1 EIA Kit can be used for how many sample of blood?

    1 answer
    1. The GLP-1 EIA Kit includes a 96-well strip plate. The assay requires the addition of a blank, six standard curve points and a positive control. It is recommend to run all standards, controls and samples in duplicate. After the addition of the blank, standards and controls, each assay can run 44 samples in duplicate.

      It is recommended to perform a 2-fold sample dilution for serum/plasma samples. The sample volume required per well is 100 ul of 1:2 diluted samples. Therefore 62.5 ul of starting sample volume is sufficient to run each sample in duplicate.

      For more information on the product , please view the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/159/844/rab0201bul.pdf

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