RAB0331

Human PSA-total ELISA Kit

for serum, plasma, cell culture supernatant and urine

• Synonym(e): Gamma-seminoprotein), Kallikrein-3, Prostate-specific antigen
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... KLK3(354)
• Input: sample type serum; sample type urine; sample type plasma; sample type cell culture supernatant(s)
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: wet ice

Eigenschaften

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type serum; sample type urine; sample type plasma; sample type cell culture supernatant(s)
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 8 pg/mL; standard curve range: 10.24-2500 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... KLK3(354)

Beschreibung

General description

The Human PSA-total (Prostate Specific Antigen, total) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme- linked immunosorbent assay for the quantitative measurement of human PSA-total in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human PSA

Application

For research use only. Not for use in diagnostic procedures.
Human PSA-total ELISA Kit has been used to determine PSA (prostate specific antigen) level in serum of prostate cancer patients and cancer-free patients.

Biochem/physiol Actions

Prostate-specific antigen (PSA) is an androgen-regulated serine protease specifically produced by prostatic tissue and is secreted into the lumen. PSA in semen cleaves semenogelins present in the seminal coagulum. Total PSA levels are increased in serum of prostate cancer (PCa) patients. PSA functions as a potential biomarker for PCa.[1] Serum protease inhibitors such as α1-antichymotrypsin (α1-ACT), α2-macroglobulin (α2-M), and other acute-phase proteins form irreversible complexes with circulating PSA and hinder proteolytic activity of PSA.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

• RABELADA

  ELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  SDB
• RABELADB

  ELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  SDB
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDB
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDB
• RABWASH4

  20X Wash Buffer (Item B)

  SDB

Sicherheitshinweise

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Lagerklasse: 10 - Combustible liquids