This kit is designed for the quantitative measurement of human VEGF in various sample types, including serum, plasma, cell culture supernatants, and urine. The kit employs an antibody specific for human VEGF coated on a 96-well plate, making it suitable for capturing and quantifying VEGF levels in samples. Usage instructions can be reviewed in the User Guide at https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/244/454/rab0507ugrev0824-ms.pdf.
Fortfahren mit
RAB0507
Human VEGF-A ELISA Kit
for serum, plasma, cell culture supernatants and urine
Aktionsangebot verfügbar
Synonym(e):
VEGF-A, Vascular endothelial growth factor A
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 kit | Versand in der Regel in 1 Woche. (Bei Bestellungen außerhalb der USA und Europas rechnen Sie bitte zusätzlich 1-2 Wochen für die Lieferung ein) | € 539,00 |
Über diesen Artikel
NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... VEGFA(7422)
Input:
sample type plasma
sample type cell culture supernatant(s)
sample type serum
sample type urine
sample type cell culture supernatant(s)
sample type serum
sample type urine
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
€ 539,00
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Versand in der Regel in 1 Woche. (Bei Bestellungen außerhalb der USA und Europas rechnen Sie bitte zusätzlich 1-2 Wochen für die Lieferung ein)
Technischer Dienst
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Unterstützung erhaltenspecies reactivity
human
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable, capture ELISA: suitable
input
sample type plasma
sample type cell culture supernatant(s)
sample type serum
sample type urine
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-6000 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... VEGFA(7422)
General description
The Human VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human VEGF in serum, plasma, cell culture supernatants and urine.
Immunogen
Recombinant Human VEGF-A
Application
For research use only. Not for use in diagnostic procedures.
Human VEGF (vascular endothelial growth factor) ELISA Kit has been used to measure VEGFA levels in human umbilical vascular endothelial cells (HUVECs) incubated with plasma from pre-eclamptic and healthy pregnant women.
Human VEGF (vascular endothelial growth factor) ELISA Kit has been used to measure VEGFA levels in human umbilical vascular endothelial cells (HUVECs) incubated with plasma from pre-eclamptic and healthy pregnant women.
Biochem/physiol Actions
VEGF (vascular endothelial growth factor) is a potent angiogenic cytokine. It stimulates proliferation and migration of endothelial cells. VEGF induces microvascular permeability by enhancing function of vesicular-vacuolar organelle (VVO). VEGF plays a prominent role in both normal and pathological angiogenesis. Substantial evidence suggests that VEGF is involved in the induction of tumor metastasis and intraocular neovascular syndromes. Increased expression of VEGF causes retinal angiogenesis, thus, inhibition of VEGF using soluble VEGF-receptor chimeric proteins can be considered as a potential therapy for ischemic retinal disease. Polymorphism in VEGF at C (-634) G is linked with diabetic retinopathy in type 2 diabetes. Altered expression of VEGF is also associated with rheumatoid arthritis, Graves′ disease, psoriasis, cardiovascular disease, and peripheral limb disease.
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
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Dieser Artikel | |||
|---|---|---|---|
| description for serum, plasma, cell culture supernatants and urine | description for serum, plasma, cell culture supernatant and urine | description for serum, plasma, cell culture supernatant and urine | description for serum, plasma, cell culture supernatant, urine |
| species reactivity human | species reactivity human | species reactivity human | species reactivity human |
| Gene Information human ... VEGFA(7422) | Gene Information human ... VEGFC(7424) | Gene Information human ... FIGF(2277) | Gene Information human ... FLT4(2324) |
| assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% |
| input sample type plasma | input sample type cell culture supernatant(s) | input sample type cell culture supernatant(s) | input sample type serum |
| technique(s) capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) capture ELISA: suitable | technique(s) capture ELISA: suitable |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
Kit-Komponenten auch einzeln erhältlich
Produkt-Nr.
Beschreibung
SDB & Preisangaben
Signalwort
Warning
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Met. Corr. 1
Lagerklasse
10 - Combustible liquids
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With this product, can I measure controlled liberation of VEGF in vitro?
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