S0677

Streptavidin aus Streptomyces avidinii

recombinant, expressed in E. coli, lyophilized powder

• CAS-Nummer: 9013-20-1
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00082035
• eCl@ss: 32160702
• Form: lyophilized powder
• Recombinant: expressed in E. coli

Eigenschaften

• Quality Segment: 200
• recombinant: expressed in E. coli
• form: lyophilized powder
• capacity: 4 mol/mol binding capacity (Biotin)
• storage temp.: −20°C

Beschreibung

General description

Streptavidin is a biotin-binding protein that is obtained from Streptomyces avidinii. It is resistant to extreme temperature, pH, detergents and proteolytic enzymes. Streptavidin has a molecular weight of 60kDa and exists as a homotetramer.

Application

Streptavidin from Streptomyces avidinii has been used-

• for blocking of the AFM (atomic force microscopy) tip, during the measurement of unbinding forces in the avidin-biotin system
• for coupling of anti-HA (hemagglutinin) antibodies during cell free synthesis and assembly of proteins on a biochip
• for the coating of the gelatin flow phantoms to eventually determine the feasibility of IVUS (intravascular ultrasound) transducer for (biotinylated) microbubble-based drug delivery
• to immobilize proteins and oligonucleotides

Biochem/physiol Actions

Streptavidin is an antibiotic that functions by binding to and depleting the essential vitamin biotin from the surrounding environment. Because of its unique properties, streptavidin has found various applications in biological studies, including immunotherapy, immunoassays, hybridization assays, lymphocyte activation, antigen localization and affinity chromatography.

Physical form

Lyophilized from 5 mM potassium phosphate buffer

Other Notes

One unit will bind 1.0 μg biotin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)