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S9415
Streptavidin−Maleimide from Streptomyces avidinii
Synonym(e):
Streptavidin Conjugate
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
2 mg | Warenkorb auf Verfügbarkeit prüfen | € 458,00 |
5 mg | Warenkorb auf Verfügbarkeit prüfen | € 951,00 |
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Technischer Dienst
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Unterstützung erhaltenextent of labeling
4-10 mol maleimide per mol Streptavidin
Quality Segment
suitability
suitable for direct conjugation to compounds containing free sulfhydryl groups
storage temp.
−20°C
General description
Streptavidin conjugated compounds may be used in avidin/strepavidin:biotin-based detection systems.
Streptavidin is a protein produced by the bacterium Streptomyces avidinii. It has four binding sites for biotin, as does avidin. Both have been used extensively as probes in immunochemical systems, conjugated to antibodies, enzymes or fluorochromes. However, avidin is highly basic (pI ~ 10.5), compared to streptavidin (pI ~ 5-6). Avidin has a slightly higher binding capacity for biotin than streptavidin does, but it has glycosylated side chains which give higher backgrounds in blotting techniques than does streptavidin, which is not a glycoprotein. Streptavidin is more resistant than avidin to dissociation into subunits by guanidinium chloride.
Application
Maleimide Activated Streptavidin is used to prepare streptavidin-protein conjugates with proteins, peptides and other molecules that contain a free sulfhydryl (-SH) group. Activated streptavidin presents an available maleimide group that reacts with sulfhydryl-containing molecules.
Physical form
Lyophilized powder containing sodium citrate.
Preparation Note
Streptavidin, from Streptomyces avidinii, activated with maleimidocaproic acid N-hydroxysuccinimide ester.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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|---|---|---|---|
| description - | description affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein | description recombinant, expressed in E. coli, lyophilized powder | description 20 nm particle size |
| suitability suitable for direct conjugation to compounds containing free sulfhydryl groups | suitability - | suitability - | suitability - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200, 400 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| extent of labeling 4-10 mol maleimide per mol Streptavidin | extent of labeling - | extent of labeling - | extent of labeling - |
Lagerklasse
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, type N95 (US)
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