S0937

Sucrose Phosphorylase

recombinant, expressed in E. coli, lyophilized powder, ≥45 units/mg solid

• Synonym(e): SPase, disaccharide glucosyltransferase, sucrose glucosyltransferase, Sucrose:orthophosphate α-D-glucosytransferase
• CAS-Nummer: 9074-06-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 2.4.1.7 (BRENDA, IUBMB)
• MDL number: MFCD00132397
• Specific activity: ≥45 units/mg solid
• Recombinant: expressed in E. coli

Eigenschaften

• recombinant: expressed in E. coli
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥45 units/mg solid
• mol wt: 56 kDa by SDS-PAGE
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

General description

Research area: Cell signaling
Sucrose Phosphorylase belongs to glycoside hydrolase, GH13 family. It comprises of four domains with the glucose anomeric carbon-binding site and a glucoside-binding site. The active site residues include Asp192 and Glu232. It is majorly produced by bifidobacteria and lactic acid bacteria. The cross-linked sucrose phosphorylase aggregates is thermostable and could be exploited for industrial catalysis of glycosylation.

Application

Sucrose Phosphorylase has been used in sucrose determination in wheat plant and in sucrose hydrogen production.

Sucrose phosphorylase has been used:

• To assess the enzymatic synthesis of stable, odorless, and powdered furanone glucosides.
• To investigate the novel transglucosylating reaction with carboxylic compounds.
• In sucrose determination in wheat plant and in sucrose hydrogen production.

Biochem/physiol Actions

Sucrose phosphorylase catalyzes the reversible conversion of sucrose (α-D-glucopyranosyl-1,2-β-D-fructofuranoside) and phosphate into D-fructose and α-D-glucose 1-phosphate. This reaction plays a crucial role in generating the vital glucose component through sucrose metabolism.(1)

Physical form

Contains sucrose as stabilizer.

Other Notes

One unit will produce 1.0 μmole of D-fructose from sucrose per min with the corresponding reduction of NADP to NADPH at pH 7.6, at 25 °C.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3