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S7651

Sphingomyelinase from Bacillus cereus

lyophilized powder, ≥100 units/mg protein

Synonym(e):

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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Größe/SKUVerfügbarkeitPreis
10 units
Warenkorb auf Verfügbarkeit prüfen
€ 163,00
50 units
Warenkorb auf Verfügbarkeit prüfen
€ 613,00

Über diesen Artikel

CAS-Nummer:
9031-54-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-869-9
MDL number:
MFCD00132362
EG-Nummer:
3.1.4.12 (BRENDA, IUBMB)
Specific activity:
≥100 units/mg protein

€ 163,00

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Unterstützung erhalten

form

lyophilized powder

Quality Segment

specific activity

≥100 units/mg protein

mol wt

36873 Da

composition

Protein, ~10% Lowry

storage temp.

2-8°C

Gene Information

Bacillus cereus ATCC 14579 ... BC0671(1203022)

General description

Sphingomyelinase modulates adenosine triphosphate (ATP)-independent endocytosis of fibroblast and large vesicles of macrophages. Sphingomyelinase causes hemolysis of human erythrocytes. It is also a potent virulence factor leading to invasive diseases.
Sphingomyelinase from Bacillus cereus (Bc-Smase) is a Mg2+-containing metalloenzyme. Bacillus cereus sphingomyelinase induces hemolysis of sheep erythrocytes.

Application

Sphingomyelinase from Bacillus cereus has been used:
  • in the measurement of membrane capacitance in brain slices
  • for testing and confirming its silkworm-killing activity
  • in enzyme activity assay and for elucidating its effect on hydrolysis of sphingomyelin in complex lipid mixtures

Sphingomyelinase has been used in a study to investigate skeletal manifestations in pediatric and adult patients with Niemann Pick disease.

Biochem/physiol Actions

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Physical form

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.

Other Notes

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

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Dieser Artikel
S9396S8633P5542
Sphingomyelinase from Bacillus cereus lyophilized powder, ≥100 units/mg protein

Sigma-Aldrich

S7651

Sphingomyelinase from Bacillus cereus

Sphingomyelinase from Bacillus cereus buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

Sigma-Aldrich

S9396

Sphingomyelinase from Bacillus cereus

Sphingomyelinase aus Staphylococcus aureus buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)

Sigma-Aldrich

S8633

Sphingomyelinase aus Staphylococcus aureus

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus lyophilized powder, ≥1,000 units/mg protein (Lowry)

Sigma-Aldrich

P5542

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

description

lyophilized powder, ≥100 units/mg protein

description

buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

description

buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)

description

lyophilized powder, ≥1,000 units/mg protein (Lowry)

specific activity

≥100 units/mg protein

specific activity

≥100 units/mg protein (Lowry)

specific activity

100-300 units/mg protein (Lowry)

specific activity

≥1,000 units/mg protein (Lowry)

Gene Information

Bacillus cereus ATCC 14579 ... BC0671(1203022)

Gene Information

Bacillus cereus ATCC 10987 ... spH(2751530)

Gene Information

-

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

form

lyophilized powder

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

lyophilized powder

mol wt

36873 Da

mol wt

34.2 kDa

mol wt

-

mol wt

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

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Lagerklasse

11 - Combustible Solids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

PSA (Persönliche Schutzausrüstung)

Eyeshields, Gloves, type N95 (US)

Piktogramme

Health hazard
GHS08

Signalwort

Danger

Gefahrencodes

H334

Sicherheitshinweise

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

WGK

WGK 1

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Analysenzertifikate (COA)

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Questions

  1. Hi for product number S7651, Sphingomyelinase from Bacillus cereus, once prepared how long can the Sphingomyelinase solution be kept and should it be kept at 2-8C or -20C?

    1 answer

    1. This product is stable for several days when stored refrigerated. Prepared solutions in aqueous buffers containing 50 % Glycerol are stable for extended periods when refrigerated.

      To review these product options please see the link below:
      https://www.sigmaaldrich.com/search/9031-54-3?focus=products&page=1&perpage=30&sort=relevance&term=9031-54-3&type=product

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