Product S9059 was manufactured using passive conjugation of the STP with 10 nm gold colloid. It does not involve covalent or linker chemistry. The protein binds to the gold colloid through amino acid residues, including charge attraction (lysine), hydrophobic attraction (tryptophan), and sulfur bonding (cysteine).
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S9059
Streptavidin−Gold from Streptomyces avidinii
~10 nm nominal (colloidal gold), buffered aqueous glycerol solution
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
0.25 mL | Warenkorb auf Verfügbarkeit prüfen | € 189,00 |
1 mL | Warenkorb auf Verfügbarkeit prüfen | € 485,00 |
Über diesen Artikel
Conjugate:
gold conjugate
Species reactivity:
-
Application:
—
Citations:
1
€ 189,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenconjugate
gold conjugate
Quality Segment
form
buffered aqueous glycerol solution
concentration
~2.5 A520 units/mL
nominal
~10 nm (colloidal gold)
mean particle size
8-12 nm (monodisperse)
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Strepavidin-collodial gold conjugate binds to molecules, such as primary antibodies, that have been previously conjugated with biotin.
Streptavidin is a protein produced by the bacterium Streptomyces avidinii. It has four binding sites for biotin as does avidin. Both have been used extensively as probes in immunochemical systems, conjugated to antibodies, enzymes, fluorochromes and gold. Avidin is highly basic (pI ~ 10.5), compared to streptavidin (pI ~ 5-6). Avidin has a slightly higher binding capacity for biotin than streptavidin does, but it has glycosylated side chains which give higher backgrounds in blotting techniques than does streptavidin, which is not a glycoprotein. Streptavidin is more resistant than avidin to dissociation into subunits by guanidinium chloride. Streptavidin may be conjugated to colloidal gold.
Application
For detection of biotinylated compounds.
Strepavidin-collodial gold conjugate is a component of streptavidin/colloidal gold-biotin detection systems use in immunoblotting, light microscopy, and immunoelectron microscopy applications. It is commonly used in postembedding immunocytochemistry.
Physical form
Suspension in 0.01 M phosphate buffered saline, pH 7.2, containing 1% BSA, 20% glycerol, and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Dieser Artikel | |||
|---|---|---|---|
| description ~10 nm nominal (colloidal gold), buffered aqueous glycerol solution | description essentially salt-free, lyophilized powder, ≥13 units/mg protein | description essentially salt-free, lyophilized powder, ≥5 units/mg protein | description affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein |
| conjugate gold conjugate | conjugate - | conjugate FITC conjugate | conjugate - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| form buffered aqueous glycerol solution | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form powder |
| concentration ~2.5 A520 units/mL | concentration - | concentration - | concentration - |
| mean particle size 8-12 nm (monodisperse) | mean particle size - | mean particle size - | mean particle size - |
| nominal ~10 nm (colloidal gold) | nominal - | nominal - | nominal - |
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Lagerklasse
10 - Combustible liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Can you provide details on how streptavidin is conjugated to gold nanoparticles? Specifically, I'd like to know if there is any covalent binding involved or if it's a passive binding process.
1 answer-
Helpful?
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I want to know the streptavidin concentration in this stock. (e.g. 0.5 mg/mL)
1 answer-
This information is reported as the protein concentration in the lot-specific Certificate of Analysis. A review of lots available in the last 7 years reveal a range of 11 - 11.6 ug/mL. Please see the link below to review a sample or lot-specific Certificate of Analysis:
https://www.sigmaaldrich.com/US/en/product/sigma/s9059#product-documentationHelpful?
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