S9655

Active Recombinant Human Superoxide Dismutase

Lyophilized powder, >20,000U/mg protein

• Synonym(e): SOD1, superoxide dismutase 1, Cu/Zn superoxide dismutase, 5-LO, Cu/Zn SOD, Human, Recombinant, E. coli
• UNSPSC Code: 12352204
• Form: lyophilized powder
• Biological source: human
• Mol wt: 16 kDa

Eigenschaften

• biological source: human
• Quality Segment: 100
• form: lyophilized powder
• specific activity: ≥20000 U/mg
• mol wt: 16 kDa
• purified by: chromatography
• suitability: suitable for molecular biology
• UniProt accession no.: P00441
• shipped in: wet ice
• storage temp.: -10 to -25°C
• Gene Information: human ... SOD1(6647)

Beschreibung

General description

Superoxide Dismutase is a metalloenzyme that catalyzes the dismutation of superoxide radicals into molecular oxygen and hydrogen peroxide. Multiple isoforms exist in mammalian systems, including cytosolic Cu/Zn-SOD (SOD1), mitochondrial Mn-SOD (SOD2), and extracellular SOD (SOD3), each differing in metal cofactor and subcellular localization. SOD plays a fundamental role in cellular defense against oxidative stress and is essential for maintaining redox balance.

Application

Superoxide dismutase has been used in:

• Antioxidant activity assays
• Oxidative stress and ROS studies
• Cell protection and apoptosis studies
• Drug screening and toxicity studies

Biochem/physiol Actions

SOD catalyzes the conversion of superoxide anions (O₂⁻) into hydrogen peroxide (H₂O₂) and molecular oxygen, thereby protecting cells from oxidative damage. It plays an essential role in maintaining redox homeostasis and preventing cellular injury.

Physical form

20mM PB (pH 7.0), 0.1M NaCl

Centrifuge the vial at 4ºC before opening to recover the entire contents. It is recommended that sterile water be added to the vial to prepare a stock solution.

Preparation Note

Centrifuge the vial at 4ºC before opening to recover the entire contents. It is recommended that sterile water be added to the vial to prepare a stock solution.

Unit definition: One unit of SOD is defined as the amount of enzyme causing half the maximum inhibition of the oxidation of 7.5 mM NADH in the presence of EDTA, manganese ions, and mercaptoethanol at 23°C and pH 7.4 in lnvitrogen Superoxide Dismutase (SOD) Colorimetric Activity Kit (Cat# EIASODC)

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids