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SAE0200
MycoPolyzyme
for use with yeast and fungal lysis, free of DNA contaminants, suitable for Microbiome research
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
20 mg | Warenkorb auf Verfügbarkeit prüfen | € 276,00 |
Über diesen Artikel
NACRES:
NA.77
UNSPSC Code:
12352204
€ 276,00
Warenkorb auf Verfügbarkeit prüfen
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Unterstützung erhaltenGeneral description
Lytic Enzyme Mixture is a mix of two enzymes: lyticase and chitinase, which have been proven useful in fungal and yeast lysis for microbiome studies. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation. Chitinases have been detected in many microorganisms and in plants. In fungi, chitinases assist in morphogenesis, to break down the inherent chitin content of fungal cell walls. Plant chitinases help in resistance to fungal attack and counteracting fungal growth, by targeting those same fungal cell walls. In bacteria, bacterial chitinases assist in utilizing chitin as a carbon source and as an energy source. The mixture of the two enzymes has also been tested to ensure absence of microbial DNA.
Application
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. Lytic Enzyme Mixture undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Other Notes
The enzymes in Lytic Enzyme Mixture are:
The enzymes are tested for absence of contaminating microbial DNA using 16S and 18S PCR amplification.
- Lyticase
- Chitinase
The enzymes are tested for absence of contaminating microbial DNA using 16S and 18S PCR amplification.
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Dieser Artikel | |||
|---|---|---|---|
| description for use with yeast and fungal lysis, free of DNA contaminants, suitable for Microbiome research | description Suitable for Microbiome research, lyophilized powder | description for use with fungal and yeast extracts, DMSO solution | description lyophilized powder (essentially salt free), ≥200 units/g solid |
| form lyophilized powder | form lyophilized powder | form DMSO solution | form lyophilized powder (essentially salt free) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in - |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 300 |
| feature DNA free | feature DNA free | feature - | feature - |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
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