SML4176

MCU-i4

≥98% (HPLC), powder, mitochondrial calcium uniporter blocker

• Synonym(e): 4-[[4-(Diethylamino)phenyl]amino]-6-methyl-3-quinolinecarboxylic acid ethyl ester, Ethyl 4-[[4-(diethylamino)phenyl]amino]-6-methyl-3-quinolinecarboxylate, MCU i4, MCUi4
• Empirische Formel (Hill-System): C₂₃H₂₇N₃O₂
• CAS-Nummer: 371924-24-2
• Molekulargewicht: 377.48
• MDL number: MFCD02730422
• NACRES: NA.21
• UNSPSC Code: 12352200
• Assay: ≥98% (HPLC)
• Form: powder

Eigenschaften

• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: powder
• color: faint green to dark yellow
• solubility: DMSO: 2 mg/mL, clear (warmed)
• storage temp.: -10 to -25°C
• SMILES string: O=C(C1=CN=C2C=CC(C)=CC2=C1NC3=CC=C(N(CC)CC)C=C3)OCC

Beschreibung

Application

MCU-i4 may be used as a mitochondrial calcium uniporter (MCU) inhibitor to study its effects on cell viability, apoptosis, cytosolic Ca²⁺ levels, mitochondrial Ca²⁺ and membrane potential, glycolytic rate, ATP production, and reactive oxygen species generation in breast cancer cells.

Biochem/physiol Actions

MCU-i4 is a mitochondrial calcium uniporter (MCU) inhibitor that selectively suppresses MCU complex-mediated mitochondrial Ca2+ uptake via direct interaction with MICU1. MCU-i4 (10 μM) effectively inhibits agonist-induced mitochondria Ca2+ uptake in intact cells (agonist = 100 μM histamine when using HeLa, 100 μM ATP when using MEF, MDA-MB-231, and HEK293A), exhibiting no effect in cells expressing MICU1 mutant construct (Q302A, Q306A, L443A) that does not bind MCU-i4 or in MICU1-, but not MICU2-, silenced cells.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• Gefahrencodes: H302
• Sicherheitshinweise: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Lagerklasse: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable