SML4328

Mito-HE chloride

≥80% (HPLC)

• Synonym(e): Mito-hydroethidine chloride hydrochloride, [6-(3,8-diamino-6-phenyl-5(6H)-phenanthridinyl)hexyl]triphenylphosphonium chloride hydrochloride
• Empirische Formel (Hill-System): C₄₃H₄₃N₃P·Cl · xHCl
• Molekulargewicht: 668.25 (free base basis)
• UNSPSC Code: 12352200
• Assay: ≥80% (HPLC)
• Form: powder
• Storage condition: desiccated

Eigenschaften

• Quality Segment: 100
• assay: ≥80% (HPLC)
• form: powder
• storage condition: desiccated
• color: faint orange to red
• solubility: DMSO: 2 mg/mL, clear
• shipped in: dry ice
• storage temp.: −70°C

Beschreibung

Biochem/physiol Actions

Mito-HE is a mitochondria superoxide probe that corresponds to dihydroethidium covalently linked to the mitochondria-targeting triphenylphosphonium (TPP+) cation. Mito-HE is widely used for live cell imaging. Cells are incubated with the probe at a final concentration of 0.3-5 μM for 10-30 min at 37°C before washing. Two different excitation wavelengths are used to distinguish the superoxide-dependent hydroxylation of Mito-HE (385-405 nm) from the nonspecific formation of ethidium (480-520 nm). DMSO stock solutions should be stored at -20°C for no more than 3 weeks. Please refer to Nat Protoc. 2008 3(6):941-7 (PMID 18536642) for experimental setup recommendations.

Disclaimer

Hygroscopic, store with desiccant

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• Gefahrencodes: H302,H315,H319
• Sicherheitshinweise: P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
• Lagerklasse: 11 - Combustible Solids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable