SML4419

ALKBH5 Inhibitor, Compound 20m

≥97% (HPLC)

• Synonym(e): 5-Hydroxy-1-(3-(trifluoromethyl)phenyl)-1H-pyrazole-3-carboxylic acid, ALKBH5i-Compound 20m, Compound 20m
• Empirische Formel (Hill-System): C₁₁H₇F₃N₂O₃
• CAS-Nummer: 1505579-77-0
• Molekulargewicht: 272.18
• UNSPSC Code: 12352200
• eCl@ss: 32150313
• Assay: ≥97% (HPLC)
• Form: powder

Eigenschaften

• Quality Segment: 100
• assay: ≥97% (HPLC)
• form: powder
• color: white to beige
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: -10 to -25°C

Beschreibung

Biochem/physiol Actions

Compound 20m potently inhibits RNA N⁶-methyladenosine (m⁶A) demethylase ALKBH5, with a fluorescence polarization assay IC₅₀ of 21 nM. Demonstrating high ALKBH5 selectivity, 20m shows minimal activity against FTO, ALKBH1, and ALKBH2 (IC₅₀ > 25 μM in DSF and FP assays). Cellular assays in HepG2 cells confirm 20m′s cell permeability and on-target demethylase activity, evidenced by ALKBH5 stabilization in cellular thermal shift assay and dose-dependent elevation of intracellular m⁶A levels. Molecular docking suggests inhibition occurs via 2-oxoglutarate binding pocket engagement, involving Mn(II) coordination, hydrogen bonds, and hydrophobic interactions.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• Gefahrencodes: H302,H315,H319
• Sicherheitshinweise: P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
• Lagerklasse: 11 - Combustible Solids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable