SML4442

5-TAMRA Azide

≥98% (HPLC)

• Synonym(e): N-(3-Azidopropyl)-3′,6′-bis(dimethylamino)-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-5-carboxamide, TAMRA Azide 5-isomer
• Empirische Formel (Hill-System): C₂₈H₂₈N₆O₄
• CAS-Nummer: 1557372-54-9
• Molekulargewicht: 512.56
• UNSPSC Code: 12352200
• Assay: ≥98% (HPLC)
• Form: powder
• Storage condition: desiccated, protect from light

Eigenschaften

• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: powder
• storage condition: desiccated, protect from light
• color: red to very dark purple
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: -10 to -25°C

Beschreibung

Biochem/physiol Actions

5-TAMRA Azide is a photostable, azide-functionalized carboxy-tetramethylrhodamine reporter that enables specific covalent labeling of alkyne-tagged biomolecules via bioorthogonal click chemistry. Its utility spans both copper-catalyzed (CuAAC) and copper-free strain-promoted (SPAAC) cycloadditions, facilitating applications from fixed-sample proteomics to real-time live-cell imaging. The probe leverages a bright TAMRA fluorophore (λex/λem ≈ 546/579 nm; ε ≈ 91,000 M⁻¹cm⁻¹; Φ ≈ 0.4) that maintains stable fluorescence from pH 4–9, ensuring robust quantification in fluorescence microscopy, flow cytometry, and in-gel scanning. Its pivotal advantage is the fusion of high-fidelity bioorthogonal reactivity with an orange-red spectral profile ideal for multiplexed analysis, minimizing interference from cellular autofluorescence and crosstalk with common blue and green fluorescent channels.

Disclaimer

Hygroscopic, Store with desiccant

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• Gefahrencodes: H302
• Sicherheitshinweise: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Lagerklasse: 11 - Combustible Solids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable