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TOX6

In Vitro Toxicology Assay Kit, Sulforhodamine B based

Synonym(e):

biomass viability assay, sulforhodamine B, total biomass assay

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Größe/SKUVerfügbarkeitPreis
1 kit
Warenkorb auf Verfügbarkeit prüfen
€ 361,00

Über diesen Artikel

NACRES:
NA.84
UNSPSC Code:
12352207

€ 361,00


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form

liquid

Quality Segment

usage

 kit sufficient for 1,000 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

λmax

565 nm

application(s)

cell analysis
detection

detection method

colorimetric

storage temp.

room temp

General description

The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.

Application

In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein. It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines. Absorbance of dye is measured at a wavelength of 565nm.

Biochem/physiol Actions

Dye binds to cellular protein and is then solubilized in base.

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TOX1TOX7TOX8
description

-

description

-

description

-

description

-

storage temp.

room temp

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

pkg of 1 kit

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric, fluorometric

storage condition

dry at room temperature

storage condition

dry at room temperature

storage condition

dry at room temperature

storage condition

dry at room temperature

application(s)

cell analysis
detection

application(s)

cell analysis
detection

application(s)

cell analysis
detection

application(s)

cell analysis
detection

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


Signalwort

Danger

WGK

WGK 2

Piktogramme

CorrosionEnvironment

Gefahrencodes

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A

Lagerklasse

8A - Combustible corrosive hazardous materials



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Analysenzertifikate (COA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  
  1. I would like to know if this test comes with the base for use in the assay?

    1 answer
    1. Yes, this kit includes a 125 mL bottle of 10 mM Tris base solution required as a solublization reagent.

      Please see the link below to review additional information included in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/131/762/tox6bul.pdf

      Helpful?

  2. Can you use Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based with suspension cells?

    1 answer
    1. Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B can be used with both adherent and suspension culture.The advantage of layering the fixative directly onto the media is that this allows the assay to work for cells in suspension.  Otherwise, you need to centrifuge, remove the media and then fix.  Also, it is possible to loose cells when you remove the media. It was found that adding the fixative works for the assay and the protocol was changed in 2003.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. When using Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based, what happens if I add kit component Product T5413, 10 mM Tris Base Solution, prior to fixing the cells?

    1 answer
    1. If the 10 mM Tris Base Solution, Product T5413 is added to the cells before the TCA (fixative), the cells will be lysed.  This reagent is not buffered and is not isotonic. The assay cannot be rescued if this occurs.

      Helpful?

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