TP0010

FLAG^® HA Tandem Affinity Purification Kit

• Synonym(e): Anti-ddddk, Anti-dykddddk
• NACRES: NA.32
• UNSPSC Code: 12352200

Eigenschaften

• technique(s): affinity chromatography: suitable
• storage temp.: 2-8°C

Beschreibung

General description

TAP technology is a recent development in protein purification. This technology incorporates tandem-linked affinity tags into genes of interest so that high purity fusion proteins can be isolated through two consecutive affinity purification steps. The technology is particularly useful for the isolation and identification of protein complexes by adding a tandem affinity tag to a known "bait" protein to pull down endogenous proteins that interact with the targeted protein of interest.

Application

The FLAG^® HA Tandem Affinity Purification Kit is designed for the isolation of high purity FLAG-HA dual-tagged fusion proteins from complex matrix, such as cell lysates and tissue homogenates. The kit is particularly suitable for isolation of protein complexes using TAP (Tandem Affinity Purification) technology.

Sutitable for mass spectrometry analysis and western blotting.

Learn more product details in our FLAG^® application portal.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit-Komponenten auch einzeln erhältlich

• F2426

  EZview^™ Red ANTI-FLAG^® M2 Affinity Gel, clone M2

  SDB
• A2095

  Monoclonal Anti-HA−Agarose antibody produced in mouse, clone HA-7, purified immunoglobulin, PBS suspension

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• R0278

  RIPA Buffer

  SDB
• F4799

  3X FLAG^® Peptide, lyophilized powder

  SDB
• U4883

  Urea, 8 M (after reconstitution with 16 mL high purity water)

  SDB

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• pictograms: GHS07
• signalword: Danger
• hcodes: H319,H412,EUH430
• pcodes: P201 - P202 - P273 - P391 - P405 - P501
• Hazard Classifications: Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2