TP0200

Gesamtprotein-Kit, Micro-Lowry, Onishi & Barr Modifizierung

sufficient for ~50 manual assays

• Synonym(e): Total Protein Assay Kit
• NACRES: NA.32
• UNSPSC Code: 12352202

Eigenschaften

• description: wavelength 500-750nm
• Quality Segment: 200
• usage: sufficient for ~50 manual assays
• protein detection level measuring range: 0-1 mg/mL
• parameter: 18-26 °C temperature, 200 μL sample volume
• storage temp.: 2-8°C

Beschreibung

General description

The biuret and Lowry procedures are used for protein determination. The former is widely used for clinical assays. The latter, though more sensitive, is used for investigative work and is limited by poor stability of combined reagents, non-reproducibility of color, especially at low protein concentration, and nonlinear chromogenic response with protein concentration. Ohnishi and Barr modified the biuret reagent for the Lowry procedure, thereby simplifying it while improving the stability of the combined reagent.

Application

Total Protein Kit, Micro-Lowry, Onishi & Barr Modification has been used to determine total soluble protein concentrations:

• in the dialyzates/extracts of gliadins
• in the extracts of flour (Avena sativa and Triticum durum) and seeds (Chenopodium quinoa, Salvia hispanica L.)
• in the stored samples of rat liver microsomal fractions

Biochem/physiol Actions

Colorimetric, Endpoint.assay. Ohnishi and Barr′s modification of micro Lowry method. According to procedure, dilute biuret reagent reacts with peptide bonds to yield a purple-blue complex, the color of which is intensified by the addition of phenol reagent. Absorbance, read at 550-750 nm, is used to determine results from a standard curve.

Kit-Komponenten auch einzeln erhältlich

• B3934

  Biuret reagent, protein detection level 150-1,000 μg/mL 110 mL

  SDB
• Folin & Ciocalteu’s phenol reagent 5 mL

  SDB

Sicherheitshinweise

• pictograms: GHS05
• signalword: Danger
• hcodes: H290,H314
• pcodes: P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1
• Lagerklasse: 8A - Combustible corrosive hazardous materials
• flash_point_f: Not applicable
• flash_point_c: Not applicable