X4500

Xanthinoxidase aus Kuhmilch

Grade III, ammonium sulfate suspension, ≥0.8 units/mg protein

• Synonym(e): XOD, Xanthine:oxygen oxidoreductase
• CAS-Nummer: 9002-17-9
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-657-6
• MDL number: MFCD00082145
• EG-Nummer: 1.17.3.2.
• Specific activity: ≥0.8 units/mg protein
• Biological source: bovine milk

Eigenschaften

• biological source: bovine milk
• Quality Segment: 200
• type: Grade III
• form: ammonium sulfate suspension
• specific activity: ≥0.8 units/mg protein
• technique(s): inhibition assay: suitable
• color: faint brown to dark brown
• UniProt accession no.: A0A3Q1LW91, A0A3Q1MM92, A0A3S5ZPW7
• foreign activity: uricase ≤0.1%
• storage temp.: 2-8°C

Beschreibung

General description

Research area: Cell Cycle

Xanthine Oxidase (XO) belongs to the class of complex metalloflavoproteins. It is produced by oxidation of sulfhydryl residues or by proteolysis of xanthine dehydrogenase (XDH). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.

Frühere EG-Nummer 1.1.3.22

Application

Xanthine Oxidase from bovine milk has been used:

• as a source for superoxide generation
• for the degradation of hypoxanthine
• for the detection of reactive oxygen species (ROS) in lipid model by electron spin resonance (ESR) spin trap method
• as a constituent of assay mixture in xanthine oxidase inhibition assay

Biochem/physiol Actions

Xanthine Oxidase (XO)catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid. Xanthine oxidase activity is inhibited by folic acid. [7] and various other inhibitors including 3,4-Dihydroxy-5-nitrobenzaldehyde (DHNB), febuxostat and allopurinol. XO and xanthine dehydrogenase (XDH) plays a vital role in the last two steps in the formation of urate. Elevated levels of XO has been observed in the serum of chronic liver disease patients. Therefore, XO can be used as a biomarker for the detection of liver disease.

Xanthine oxidase activity is inhibited by folic acid.

Xanthinoxidase ist ein molybdänhaltiges Enzym, das im Zytosol vorkommt und durch Flavonoide stark gehemmt werden kann. Es spielt eine wesentliche Rolle beim Metabolismus einiger Arzneimittel sowie von Purinen und Pyrimidinen. Es ist auch eine biologische Quelle für reaktive Sauerstoffspezies.

Physical form

Suspension in 2.3 M (NH₄)₂SO₄, 10 mM Natriumphosphat-Puffer, pH 7.8, enthält 1 mM EDTA und 1 mM Natriumsalicylat

Preparation Note

Chromatographisch aufgereinigt

Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

Analysis Note

Proteingehalt mittels Biuret-Reaktion bestimmt.

Other Notes

1 U setzt 1.0 μmol Xanthin zu Harnsäure pro Minute um bei pH 7.5 und 25°C. Ca. 50% der Aktivität wird erhalten mit Hypoxanthin als Substrat.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)