Z-2139

ZytoLight^® SPEC FOXO1 Dual Color Break Apart Probe

green to orange, pkg of 0.05 mL

• Synonym(e): FISH probe, dual color FISH probe

Eigenschaften

• packaging: pkg of 0.05 mL, pkg of 5 tests
• storage condition: protect from light
• color: green to orange
• application(s): research use
• storage temp.: 2-8°C

Beschreibung

General description

The ZytoLight^® SPEC FOXO1 Dual Color Break Apart Probe (PL96) is a direct-labeled FISH probe for the qualitative detection of translocations of the FOXO1 gene at 13q14.11 in formalin-fixed, paraffin-embedded specimens using fluorescence in situ hybridization (FISH). The probe is intended for use with the ZytoLight FISH-Tissue Implementation Kit.

ZytoLight^® probes enable the precise identification of genetic abnormalities, including translocations, deletions, amplifications, and chromosomal aneuploidies, in formalin-fixed, paraffin-embedded (FFPE) tissue sections or cytology specimens, without the need for additional antibody-based detection.

Components

The ZytoLight SPEC FOXO1 Dual Color Break Apart Probe comprises:

• ZyGreen (excitation 503 nm/emission 528 nm) labeled polynucleotides (~10 ng/µl), targeting sequences mapping in 13q14.11* (chr13:40,285,558-41,132,595) proximal to the FOXO1 breakpoint region;
• ZyOrange (excitation 547 nm/emission 572 nm) labeled polynucleotides (~4.5 ng/µl), targeting sequences mapping in 13q14.11* (chr13:41,246,917-42,054,781) distal to the FOXO1 breakpoint region;
• Formamide based hybridization buffer

*according to Human Genome Assembly GRCh37/hg19


Principle

The fluorescence in situ hybridization (FISH) method enables the identification and visualization of particular nucleic acid sequences within cell samples. DNA fragments labeled with fluorescent dyes, known as FISH probes, are co-denatured with their complementary target DNA strands in the sample and then allowed to hybridize. Following hybridization, non-specific and unbound probe fragments are removed through stringency washes. The DNA is then counterstained with DAPI, and the hybridized probes are detected using a fluorescence microscope equipped with filters matched to the specific fluorochromes used to label the FISH probes.

Application

This probe is intended for use in formalin-fixed, paraffin-embedded specimens to qualitatively detect translocations of the FOXO1 gene at 13q14.11, via FISH.

Analysis Note

Using suitable filter sets, the hybridization signals of the probe appear green near the FOXO1 breakpoint region and orange away from the FOXO1 breakpoint region.

• Normal scenario: in normal cells during interphase or cells without a translocation of the FOXO1 gene region, two green/orange fusion signals are observed.
• Aberrant scenario: one translocated FOXO1 gene region is shown by one separate green and orange signal each.

Overlapping signals may appear yellow.

Legal Information

ZytoLight is a registered trademark of ZytoVision GmbH in Germany

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H351,H360D,H373
• pcodes: P202 - P260 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 2 - Repr. 1B - STOT RE 2 Oral
• target_organs: Blood
• Lagerklasse: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• flash_point_f: Not applicable
• flash_point_c: Not applicable