The oxysterol and cholestenoic acid profile of mouse cerebrospinal fluid.

The oxysterol and cholestenoic acid profile of mouse cerebrospinal fluid.

Steroids (2015-03-12)

Peter J Crick, Lien Beckers, Myriam Baes, Paul P Van Veldhoven, Yuqin Wang, William J Griffiths

ZUSAMMENFASSUNG

Oxysterols and cholestenoic acids are oxidised forms of cholesterol with a host of biological functions. The possible roles of oxysterols in various neurological diseases makes the analysis of these metabolites in the central nervous system of particular interest. Here, we report the identification and quantification of a panel of twelve sterols in mouse cerebrospinal fluid (CSF) using liquid chromatography-mass spectrometry exploiting enzyme assisted derivatisation for sterol analysis technology. We found low levels of oxysterols and cholestenoic acids in CSF in the range of 5pg/mL-2.6ng/mL. As found in man, these concentrations are one to two orders of magnitude lower than in plasma.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

I9516

Sigma-Aldrich

2-Propanol, BioReagent, ≥99.5%, Molecular Biology

271004

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Acetonitril, anhydrous, 99.8%

459836

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Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%

C8667

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Cholesterin, Sigma Grade, ≥99%

322415

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Methanol, anhydrous, 99.8%

P5655

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Kaliumphosphat, powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

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2-Propanol, anhydrous, 99.5%

493511

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Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%

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Isopropylalkohol, ≥99.7%, FCC, FG

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Cholesterin, powder, BioReagent, suitable for cell culture, ≥99%

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Kaliumphosphat, ReagentPlus^®

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2-Propanol, BioUltra, Molecular Biology, ≥99.5% (GC)

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Kaliumphosphat, Molecular Biology, ≥98.0%

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Essigsäure, suitable for luminescence, BioUltra, ≥99.5% (GC)

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2-Propanol, electronic grade, 99.999% trace metals basis

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Acetonitril, electronic grade, 99.999% trace metals basis

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5α-Androstan-17β-ol-3-on, ≥97.5%

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Kaliumphosphat, BioUltra, Molecular Biology, anhydrous, ≥99.5% (T)

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Essigsäure, ≥99.5%, FCC, FG

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Ameisensäure, ≥95%, FCC, FG

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Essigsäure, natural, ≥99.5%, FG

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Kaliumphosphat, 99.99% trace metals basis

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SAFC

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