H3784

HEPES sodium salt

BioPerformance Certified, suitable for cell culture, ≥99.0%

• Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
• Empirical Formula (Hill Notation): C₈H₁₇N₂NaO₄S
• CAS Number: 75277-39-3
• Molecular Weight: 260.29
• NACRES: NA.25
• PubChem Substance ID: 24895595
• UNSPSC Code: 12161700
• EC Number: 278-169-7
• MDL number: MFCD00036463

Properties

• Product Name: HEPES sodium salt, BioPerformance Certified, suitable for cell culture, ≥99.0%
• InChI key: RDZTWEVXRGYCFV-UHFFFAOYSA-M
• InChI: 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
• SMILES string: [Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
• grade: BioPerformance Certified
• assay: ≥99.0%
• form: powder
• storage condition: dry at room temperature
• technique(s): activity assay: suitable; cell culture | mammalian: suitable
• impurities: endotoxin and total aerobic microbial count, tested
• color: white
• pH: 10-12 (1 M in water)
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• solubility: water: 0.1 M, clear, colorless
• density: 1.19 g/cm³ at 20—25 °C; 1.21 g/cm³ at 20—25 °C
• absorption: ≤0.02 at 280 in H₂O at 0.1 M; ≤0.04 at 260 in H₂O at 0.1 M
• suitability: suitable for component for culture media; suitable for molecular biology
• application(s): cell analysis; diagnostic assay manufacturing; general analytical; life science and biopharma
• foreign activity: DNase, RNase, NICKase, protease, none detected
• Quality Level: 400

Description

Application

HEPES sodium salt has been used:

• to supplement the HSOF medium[1]
• as a buffer component of HCZB medium in egg and embryo culture[2]
• as a buffer component in cell culture[3]

Features and Benefits

• High-purity product for biochemical and biological research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
• Suitable for Cell culture
• Tested for Endotoxins and Total Aerobic Microbial Count
• Free from DNase, NICKase, RNase, Endonuclease, Exonuclease and Protease

General description

HEPES, or 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, is a zwitterionic N-substituted aminosulfonic acid buffer with a remarkable pH buffering capacity between 6.8 and 8.2 (pKa of 7.48 at 25°C). Widely recognized for its versatility, HEPES is extensively employed in cell biology, biochemical, and biological research. Notably, its neutrality towards metal ions makes it a preferred choice for solutions with metal ions, acting as a "non-coordinating buffer."

In cell culture, HEPES excels in maintaining stable physiological pH even amidst fluctuating carbon dioxide concentrations, outperforming bicarbonate buffers (NaHCO₃). Moreover, HEPES showcases valuable properties in various biological and biochemical processes, such as isoelectric focusing for protein separation and minimal interference in DNA-restriction enzyme reactions, making it indispensable across diverse research applications, including immunoprecipitation, cell lysis, and live cell imaging. Its versatility, exceptional buffering capacity, and minimal interaction with molecules establish HEPES as a key tool in various research domains.

Other Notes

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Physical form

Lyophilized, spray dried, or crystallized material.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)