D9170

Dimethyl sulfoxide

PCR Reagent

• Synonym(s): DMSO
• Linear Formula: (CH₃)₂SO
• CAS Number: 67-68-5
• Molecular Weight: 78.13
• UNSPSC Code: 12191502
• NACRES: NA.25
• PubChem Substance ID: 24894271
• EC Number: 200-664-3
• Beilstein/REAXYS Number: 506008
• MDL number: MFCD00002089
• Assay: ≥99.5%
• Technique(s): PCR: suitable
• Application(s): agriculture
• Bp: 189 °C (lit.)
• Vapor pressure: 0.42 mmHg ( 20 °C)

Properties

• Product Name: Dimethyl sulfoxide, PCR Reagent
• InChI key: IAZDPXIOMUYVGZ-UHFFFAOYSA-N
• InChI: 1S/C2H6OS/c1-4(2)3/h1-2H3
• SMILES string: CS(C)=O
• vapor density: 2.7 (vs air)
• vapor pressure: 0.42 mmHg ( 20 °C)
• assay: ≥99.5%
• form: liquid
• autoignition temp.: 573 °F
• shelf life: Recommended retest period - 2 years
• expl. lim.: 42 %, 63 °F
• packaging: vial of 1 mL
• technique(s): PCR: suitable
• impurities: ≤0.1% water; water
• color: colorless
• refractive index: n20/D 1.479 (lit.)
• bp: 189 °C (lit.)
• mp: 16-19 °C (lit.)
• solubility: H₂O: miscible (completely)
• density: 1.10 g/mL (lit.)
• suitability: suitable for PCR
• application(s): agriculture
• Quality Level: 200

Description

Application

Dimethyl sulfoxide has been used as a component of polymerase chain reaction (PCR) mixture[1] and as PCR facilitator.[2][3]

Disclaimer

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

General description

Dimethyl sulfoxide is a free radical scavenger and a cryopreservant. It elicits protective effect in double stranded breaks(DSBs).[4] Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal stability against depurination.[2] As a PCR cosolvent, DMSO may help improve yields, especially in long PCR.[5]

Other Notes

For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: 188.6 °F - closed cup
• flash_point_c: 87 °C - closed cup
• ppe: Eyeshields, Gloves, type ABEK (EN14387) respirator filter