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276855
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D8418
Dimethyl sulfoxide
Molecular Biology
Dimethyl sulfoxide
Synonym(s):
Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO
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50 ML
R$786.00
100 ML
R$1,333.00
250 ML
R$2,264.00
500 ML
R$3,330.00
1 L
R$4,441.00
R$786.00
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About This Item
Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein:
506008
EC Number:
MDL number:
UNSPSC Code:
12191502
PubChem Substance ID:
NACRES:
NA.52
Grade:
Molecular Biology
Assay:
≥99.9%
Technique(s):
DNA sequencing: suitable
PCR: suitable
transfection: suitable
PCR: suitable
transfection: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)
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grade
Molecular Biology
Quality Level
vapor density
2.7 (vs air)
vapor pressure
0.42 mmHg ( 20 °C)
product line
BioReagent
Assay
≥99.9%
form
liquid
autoignition temp.
573 °F
shelf life
Recommended retest period - 2 years
expl. lim.
42 %, 63 °F
technique(s)
DNA sequencing: suitable
PCR: suitable
transfection: suitable
impurities
≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)
color
colorless
refractive index
n20/D 1.479 (lit.)
bp
189 °C (lit.)
mp
16-19 °C (lit.)
solubility
H2O: miscible (completely)
density
1.10 g/mL (lit.)
absorption
passes test
suitability
suitable for molecular biology
foreign activity
DNase and RNase, none detected
storage temp.
room temp
SMILES string
CS(C)=O
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
InChI key
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
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This Item | 41639 | D9170 | PHR1309 |
|---|---|---|---|
| assay ≥99.9% | assay ≥99.5% (GC) | assay ≥99.5% | assay - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| grade for molecular biology | grade for molecular biology | grade PCR Reagent | grade certified reference material, pharmaceutical secondary standard |
| technique(s) DNA sequencing: suitable, transfection: suitable, PCR: suitable | technique(s) - | technique(s) PCR: suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
| solubility H2O: miscible (completely) | solubility H2O: miscible (completely) | solubility H2O: miscible (completely) | solubility - |
| form liquid | form liquid | form liquid | form - |
General description
Features and Benefits:
Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.
- Suitable for Molecular Applications
- Nuclease-Free DMSO
- DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
- Molecular Biology Grade DMSO
Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.
Application
DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in
Dimethyl sulfoxide has been used-
- polymerase chain reaction (PCR)
- amplification of cDNA libraries
- DNA sequencing
- column-loading buffers for poly(A)+ RNA selection
- buffers for the transformation of competent E. coli
- transfection protocols.
Dimethyl sulfoxide has been used-
- as an oligonucleotide solvent in array spotting[1]
- as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
- for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
- for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
- in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
Features and Benefits
This product is free from DNases, RNases, phosphatases and proteases.
Disclaimer
Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Product Information Sheet
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Is this product anhydrous?
1 answer-
Helpful?
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May I know the concentration (mg/ml) for DMSO (cat no. D8418)?
1 answer-
This product is supplied as a neat or pure liquid with a density of 1.10 g/mL and a molecular weight of 78.13 g/mol. The minimum purity is 99.9%, thus the molarity will vary slightly from lot to lot. The molarity is 14.1 M.
Helpful?
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Is Product D8418, Dimethyl sulfoxide, also compatible with PET material?
1 answer-
Yes, Dimethyl sulfoxide (DMSO) is generally considered to be compatible with Polyethylene terephthalate (PET) material. The following information can be found on the Product Data Sheet:
-Compatible: LDPE, HDPE, polypropylene, PPCO (polypropylene copolymer), polymethylpentene, nylon, teflon FEP
-Moderately compatible: polystyrene, ECTFE/ETFE
-Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonatehttps://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/944/265/d2650pis.pdf
Helpful?
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Is this product sterile?
1 answer-
This product is not sterile. To prepare a sterile filtered DMSO solution, it is recommended to use a Teflon, polypropylene, or nylon membrane. See products D2650 or D2438 for sterile DMSO product options. Please review these products at the links provided below:
https://www.sigmaaldrich.com/product/sigma/d2650
https://www.sigmaaldrich.com/product/sigma/d2438Helpful?
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Is Dimethyl sulfoxide a sterile product?
1 answer-
No, this product is not considered sterile. Product Nos. D2560 and D2438 are sterile-filtered.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Does DMSO have any synthetic utility?
1 answer-
DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.
Helpful?
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Is Product D8418, Dimethyl sulfoxide, assayed for DNase, RNase and protease?
1 answer-
Yes. It is a molecular biology grade product, and it is tested for DNase, RNase and protease. For a lot to pass none of those enzymes may be detected.
Helpful?
-
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With what materials is Product D8418, Dimethyl sulfoxide, compatible?
1 answer-
The compatibility of DMSO with various materials is listed here: Compatible: LDPE, HDPE, polypropylene, PPCO (polypropylene copolymer), polymethylpentene, nylon, PTFE (Teflon) and FEP. Moderately compatible: polystyrene, ECTFE/ETFE. Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonate.
Helpful?
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Where does DMSO come from?
1 answer-
DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.
Helpful?
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