EZBRAIN-SET

Human Amyloid β40 and Amyloid β42 ELISA Kit

measures Amyloid β40 and Amyloid β42 in 50 μL tissue or cell lysate; combo pack

• Synonym(s): Amyloid β40/β42 ELISA, Brain ELISA Kit, Human Brain Amyloid ELISA
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Product Name: Human Amyloid β40 and Amyloid β42 Brain ELISA,
• species reactivity: human
• Quality Level: 200
• packaging: kit of 2 × 96 wells
• parameter: 50 μL sample volume (Overnight assay)
• assay range: sensitivity: 6.0 pg/mL; (The lowest level of Amyloid β1-40 standard used in this assay ; 50 μL sample size); sensitivity: 8 pg/mL; (The lowest level of Amyloid ß1-42 standard used in this assay; 50 μL sample size); inter-assay cv: <10; intra-assay cv: <10; standard curve range: 16-500 pg/mL
• technique(s): ELISA: suitable
• input: sample type tissue/cellular lysate
• application(s): research use
• detection method: colorimetric (450nm/590nm)
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: human ... APP(351)

Description

Application

These assays require 50 µl of sample and are overnight assays.

Biochem/physiol Actions

The Amyloid β40 ELISA uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-40, which causes a high selectivity for Aβ 40. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44. The Amyloid β42 ELISA uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-42, which causes a high selectivity for Aβ42. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.


Used to detect/quantify: Amyloid β40 & Amyloid β42

Packaging

96-well plate

Preparation Note

Components in the kit can be stored up to 2 weeks at 2-8°C

Other Notes

Contact technical support for information

Disclaimer

For research use only. Not for use in diagnostic procedures.