11284932001

DNase I

from bovine pancreas

• Synonym(s): Dnase
• UNSPSC Code: 12352204
• EC Number: 3.1.21.1 (BRENDA, IUBMB)
• NACRES: NA.54
• Biological source: bovine pancreas

Properties

• biological source: bovine pancreas
• form: lyophilized
• packaging: pkg of 100 mg
• manufacturer/tradename: Roche
• technique(s): cell culture | mammalian: suitable
• storage temp.: 2-8°C

Description

General description

DNase I is a double-strand specific endonuclease that degrades DNA. Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease, which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[1] DNase I is one of the most well characterized endonucleases of mammalian origin.[2]

Application

DNase I has been used for tissue dissociation in conjunction with collagenases.[3][4]

Biochem/physiol Actions

During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping.[5][6][7]

Features and Benefits

• Lyophilized
• Filtered through 0.2 μm pore size membrane before lyophilization
• Glycoprotein and double-strand-specific endonuclease

Preparation Note

Activator: Bivalent metal ions (Ca²⁺, Mg²⁺)
Working concentration: 0.01 to 1 mg/ml
Note: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg²⁺.

Reconstitute the lyophylizate in sterile double-distilled water (10 mg/ml); further dilution with PBS (phosphate buffered saline), HBSS (Hank′s Balanced Salt Solution) or medium.

Other Notes

For life science research only. Not for use in diagnostic procedures.

One unit is the enzyme activity which yields an increase in absorbance of 0.001 per minute under the assay conditions.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash