Yes, this product is suitable for purifying peptides in the 1,000 to 5,000 Da range. The calibration range for this product is 1 KDa to 150 KDa.
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$3,810.00
$3,810.00
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
NB.21
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Product Name
TSKgel® G3000SWXL HPLC Column, phase diol, L × I.D. 30 cm × 7.8 mm, 5 μm particle size
material
stainless steel
Agency
suitable for USP L20
suitable for USP L59
product line
TSKgel®
manufacturer/tradename
Tosoh 08541
parameter
≤0.5 M salt concentration
0-100% aqueous soluble organic solvents
1.2 mL/min flow rate
10-30 °C temperature
72 bar max. pressure
technique(s)
HPLC: suitable
L × I.D.
30 cm × 7.8 mm
matrix
silica particle platform
matrix active group
diol phase
particle size
5 μm
pore size
250 Å
operating pH
2.5-7.5
separation technique
size exclusion (SEC)
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Related Categories
1 of 4
This Item | 808540 | 805147 | 806728 |
|---|---|---|---|
| matrix active group diol phase | matrix active group diol phase | matrix active group diol phase | matrix active group diol phase |
| particle size 5 μm | particle size 5 μm | particle size 13 μm | particle size 13 μm |
| separation technique size exclusion (SEC) | separation technique size exclusion (SEC) | separation technique size exclusion (SEC) | separation technique size exclusion (SEC) |
| L × I.D. 30 cm × 7.8 mm | L × I.D. 30 cm × 7.8 mm | L × I.D. 60 cm × 21.5 mm | L × I.D. 30 cm × 21.5 mm |
| agency suitable for USP L20, suitable for USP L59 | agency suitable for USP L20, suitable for USP L59 | agency suitable for USP L20, suitable for USP L59 | agency suitable for USP L20, suitable for USP L59 |
| matrix silica particle platform | matrix silica particle platform | matrix silica particle platform | matrix silica particle platform |
General description
Widely considered the premium columns for analyzing biopolymers by GFC, TSKgel SWXL columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution. With 5μm particles and 250Å pores, TSKgel G3000SWXL is an excellent choice for protein and large peptide separations. The fractionation range is applicable for globular protein samples up to 0.5 million Da.
Application
The TSKgel G3000SWXL columns are used: To separate monoclonal antibodies based on size exclusion.
- For size-exclusion chromatography (SEC) to analyse PEGylated proteins, such as lysozyme. The column allows separation of different PEGylated isoforms based on their size.
- To effectively separate and quantify high molecular weight species, oligomers, and monomers of IgG1 and IgG2 antibodies.
- The columns provided clear resolution and accurate quantification essential for analyzing antibody stability and aggregation behavior.
Physical form
Shipped in 0.05% NaN3 and 0.1 M Na2SO4 in 0.1 M phosphate buffer, pH 6.7.
Legal Information
TSKgel is a registered trademark of Tosoh Corporation
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Chromatograms
application for HPLC
application for HPLC
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I'm planning to purify peptides in the 1,000 to 5,000 Da range. Would this product be suitable for that application?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Can this column be used on an Aekta system (FPLC)? Or is an HPLC required?
1 answer-
This product is intended for use in HPLC. However, the item may be functional under FPLC conditions with the appropriate ÄKTA adapters.
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Dears, Please advise whether or not Pre-column is required to be used with this item (Tosoh G3000 SWXL TSK Gel 7.8 mm x 30 cm, 5 µm)
1 answer-
This product does not require a pre-column, it is used as an analytical column.
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