Analysis of volatiles from Spanish honeys by solid-phase microextraction and gas chromatography-mass spectrometry.

Analysis of volatiles from Spanish honeys by solid-phase microextraction and gas chromatography-mass spectrometry.

Journal of agricultural and food chemistry (2002-04-18)

Rosa A Pérez, Consuelo Sánchez-Brunete, Rosa M Calvo, José L Tadeo

PMID11958634

ZUSAMMENFASSUNG

Headspace solid-phase microextraction (SPME), followed by gas chromatography (GC)-mass spectrometry (MS) determination, has been used for the analysis of honey volatiles. Two SPME fibers were employed to study the composition of volatiles from various types of Spanish honeys. The best results were obtained with the Carboxen/PDMS fiber, using a homogenization time of 1 h at 70 degrees C and a sampling period of 30 min. A total of 35 compounds were detected, most of them identified by GC-MS and quantified using external standards. Differences in the composition of honey volatiles were obtained, and these results allowed the differentiation of honeys. However, further studies are necessary to confirm the utility of this technique as an alternative tool for the characterization of the floral origin of honeys.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

57298-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, plain gray hub

57348-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (2cm), needle size 24 ga, Autosampler, pk of 3, notched gray hub

57328-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (1cm), needle size 24 ga, Manual Holder, pk of 3, plain gray hub

57299-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (2cm), needle size 23 ga, Autosampler, pk of 3, notched gray hub

57329-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, plain gray hub

20358

Supelco

ORBO^™ Carboxen-564 Röhrchen, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)

20355

Supelco

ORBO^™ 78 HBr auf Carboxen^® 564 speziell gereinigt (20/45), 400/200 mg, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea

25467

Supelco

Carboxen^®-1010 PLOT Kapillar-GC-Säule, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

10478-U

Supelco

Carboxen^® Adsorptionsmittel, matrix Carboxen^® 1000, 60-80 mesh, bottle of 10 g

57318

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, black hub

25461

Supelco

Carboxen^®-1006 PLOT Kapillar-GC-Säule, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

57319

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, black hub

57295-U

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, light blue hub

24246

Supelco

Carboxen^®-1010 PLOT Kapillar-GC-Säule, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

57334-U

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Manual Holder, pk of 3, light blue hub

57335-U

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, light blue hub

23446-U

Supelco

ORBO^™-93 Sorbensröhrchen, ORBO tube I.D. × L 6 mm × 95 mm, Bed A 180 mg, Bed B 90 mg, 60/80 mesh, pkg of 25 ea

57343-U

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, black hub

24241-U

Supelco

Carboxen^®-1006 PLOT Kapillar-GC-Säule, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

28718-U

Supelco

Tenax^® TA / Carboxen^® 1018, glass TD tube, preconditioned, O.D. × L 1/4 in. × 3 1/2 in., Sealed with (Swagelok^® End-Fittings), pkg of 10 ea

57344-U

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 23 ga, Manual Holder, pk of 3, black hub

10471

Supelco

Carboxen^® Adsorptionsmittel, matrix Carboxen^® 1003, 40-60 mesh, bottle of 10 g

57914-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, Metal Alloy (2cm), needle size 23 ga, Autosampler, pk of 1, plain gray hub

54294-U

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Carboxen^® 572 for Tobacco Smoke, Adsorbent Bed Wt: 300 mg, cartridge size 3 mL, pkg of 50 ea

57912-U

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SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, Metal Alloy (1cm), needle size 23 ga, Autosampler, pk of, plain gray hub

10269

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Carboxen^® Adsorptionsmittel, matrix Carboxen^® 569, 20-45 mesh, bottle of 10 g

54041-U

Supelco

Carboxen^® 1000 Reversibles SPE-Röhrchen, 200 mg/mL, pk 30

10477-U

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Carboxen^® Adsorptionsmittel, matrix Carboxen^® 1000, 40-60 mesh, bottle of 50 g

11072-U

Supelco

Carboxen^® Adsorptionsmittel, matrix Carboxen^® 572, 20-45 mesh, bottle of 10 g

57906-U

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 85 μm CAR/PDMS, Metal Alloy (1 cm), needle size 23 ga, Autosampler, pk of 1, light blue hub