Effect of Chaenomeles sinensis Extract on Choline Acetyltransferase Activity and Trimethyltin-Induced Learning and Memory Impairment in Mice.

Effect of Chaenomeles sinensis Extract on Choline Acetyltransferase Activity and Trimethyltin-Induced Learning and Memory Impairment in Mice.

Chemical & pharmaceutical bulletin (2015-12-04)

Yoon Kyung Kwon, Soo Jung Choi, Cho Rong Kim, Jae Kyeom Kim, Hye Kyung Kim, Jong Hun Choi, Sang-Wook Song, Chang-Ju Kim, Gwi Gun Park, Cheung-Seog Park, Dong-Hoon Shin

PMID26633030

ZUSAMMENFASSUNG

The aim of this study was to search for a novel choline acetyltransferase (ChAT) activator from plants traditionally grown in Korea. An ethanol extract from Chaenomeles sinensis Koehne showed the highest ChAT-activating effect in vitro in an assay that used human neuroblastoma cells and [(14)C]acetyl-CoA. The active compound was speculated to be stearic acid methyl ester (SAME). In an in vivo experiment, C. sinensis extract and SAME improved trimethyltin (TMT)-induced deficits in learning and memory in mice as assessed by a Y-maze behavioral test and a passive avoidance test. The C. sinensis extract might attenuate the TMT-induced brain disorder. This study suggests that SAME from C. sinensis might be useful in the treatment of Alzheimer's disease.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

E7023

Sigma-Aldrich

Ethyl alcohol, Pure 200 proof, Molecular Biology

459844

Sigma-Aldrich

Ethyl alcohol, Pure, 200 proof, ACS reagent, ≥99.5%

179418

Sigma-Aldrich

Toluol, ACS reagent, ≥99.5%

493546

Sigma-Aldrich

Ethyl alcohol, Pure, 200 proof, meets USP testing specifications

459836

Sigma-Aldrich

Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%

244511

Sigma-Aldrich

Toluol, anhydrous, 99.8%

S3014

Sigma-Aldrich

Natriumchlorid, Molecular Biology, DNase, RNase, and protease, none detected, ≥99% (titration)

C1879

Sigma-Aldrich

Cholinchlorid, ≥98%

S5150

Sigma-Aldrich

Natriumchlorid -Lösung, 5 M in H₂O, BioReagent, Molecular Biology, suitable for cell culture

S7653

Sigma-Aldrich

Natriumchlorid, BioXtra, ≥99.5% (AT)

S5886

Sigma-Aldrich

Natriumchlorid, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

S8776

Sigma-Aldrich

Natriumchlorid -Lösung, 0.9% in water, BioXtra, suitable for cell culture

32249

Sigma-Aldrich

Toluol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.7% (GC)

493511

Sigma-Aldrich

Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%

E9884

Sigma-Aldrich

Ethylendiamintetraessigsäure, ACS reagent, 99.4-100.6%, powder

02870

Sigma-Aldrich

Ethanol, ACS reagent, prima fine spirit, without additive, F15 o¹

E6758

Sigma-Aldrich

Ethylendiamintetraessigsäure, anhydrous, crystalline, BioReagent, suitable for cell culture

E8008

Sigma-Aldrich

Ethylendiamintetraessigsäure -Lösung, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

02856

Sigma-Aldrich

Ethanol, purum, absolute ethanol, denaturated with 4.8% isopropanol, A15 IPA¹, ≥99.8% (based on denaturant-free substance)

59222C

SAFC

Natriumchlorid -Lösung, 5 M

C7527

Sigma-Aldrich

Cholinchlorid, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%

431788

Sigma-Aldrich

Ethylendiamintetraessigsäure, 99.995% trace metals basis

EDS

Sigma-Aldrich

Ethylendiamintetraessigsäure, BioUltra, anhydrous, ≥99% (titration)

C7017

Sigma-Aldrich

Cholinchlorid, ≥99%

320552

Sigma-Aldrich

Toluol, ACS reagent, ≥99.5%

71376

Sigma-Aldrich

Natriumchlorid, BioUltra, Molecular Biology, ≥99.5% (AT)

T25402

Sigma-Aldrich

Natrium-tetraphenylborat, ACS reagent, ≥99.5%

02857

Sigma-Aldrich

Ethanol, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL¹, ~96% (based on denaturant-free substance)

71386

Sigma-Aldrich

Natriumchlorid -Lösung, BioUltra, Molecular Biology, ~5 M in H₂O

179965

Sigma-Aldrich

Toluol, Laboratory Reagent, ≥99.3%