05-1243

Anti-Sirt1 Antibody, clone 3H10.2

clone 3H10.2, Upstate^®, from mouse

• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 3H10.2, monoclonal
• Espèces réactives: human
• Fabricant/nom de marque: Upstate^®
• Technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1
• Numéro d'accès NCBI: NM_012238
• Numéro d'accès UniProt: Q96EB6
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SIRT1(23411)

Description

Description générale

120 kDa

The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53. There are now five described human homologs of yeast Sir2, which are named SIRT1-5.
SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress. SIRT1 is a HDAC which is important in establishing repressive chromatin structures and plays a role in increasing lifespan.

Immunogène

GST fusion protein corresponding to amino acids 508-616 of human Sirt1.

Application

Anti-Sirt1 Antibody, clone 3H10.2 is a Mouse Monoclonal Antibody for detection of Sirt1 also known as Sirtuin 1, Silent mating type Information Regulation-2 & has been tested in IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Actions biochimiques/physiologiques

Human Sirt1. Does not cross react with lysates from mouse NIH/3T3 cells or rat PC12 cells.

Forme physique

Format: Purified

Protein G Purified

Purified in PBS with 0.05% Sodium Azide

Notes préparatoires

Maintain at 2-8 °C in undiluted aliquots for up to 6 months after date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Remarque sur l'analyse

routinely evaluated by immunoblot on nuclear extract from HeLa cells

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 05-707

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable