Rat collagen solution can be used as a coating for human cells, as indicated by various publications. The following publication in PubMed suggests that rat collagen can serve as a viable alternative to human collagen for various applications in cell culture and tissue engineering. https://pubmed.ncbi.nlm.nih.gov/18203067/
C3867
Rat Collagen Type I
from rat tail, liquid, 4 mg/mL, suitable for cell culture
Collagen, Type I solution from rat tail
Synonyme(s) :
Collagène from rat tail, Col1a1, Collagène provenant de queues de rats
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Collagène de type I solution from rat tail, BioReagent, suitable for cell culture, sterile-filtered
biological source
rat tail
description
Approx. 100mg/vial ((35 - 40 ml) )
sterility
sterile-filtered
product line
BioReagent
assay
>95% (SDS-PAGE)
form
liquid
mol wt
apparent mol wt 115-130 kDa by SDS-PAGE (doublet)
apparent mol wt 215-235 kDa by SDS-PAGE (doublet)
packaging
pkg of 1 vial
concentration
>0.5 mg/mL Biuret
technique(s)
cell culture | mammalian: suitable
surface coverage
6‑10 μg/cm2
impurities
Mycoplasma Test by Barile, none detected
solubility
soluble, clear to hazy, colorless (solubilized in 0.02M acetic acid)
NCBI accession no.
UniProt accession no.
binding specificity
Peptide Source: Collagen
Peptide Source: Fibronectin
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Gene Information
rat ... Col1a1(29393)
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1 of 4
Cet article | C9791 | C6745 | C8919 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility - | sterility sterile-filtered | sterility aseptically processed |
| biological source rat tail | biological source bovine (calf) skin | biological source human cell culture | biological source bovine (calf) skin |
| concentration >0.5 mg/mL Biuret | concentration - | concentration 0.3 mg/mL | concentration (0.1% solution in 0.1 M acetic acid) |
| assay >95% (SDS-PAGE) | assay - | assay - | assay - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Application
La solution de collagène de type I provenant de la queue de rat a été utilisée pour revêtir des plaques et flacons de culture cellulaire pour la lignée cellulaire HepG2[1] et les podocytes.[2] Elle a également été utilisée comme étalon pour créer une courbe d′étalonnage du contenu total en collagène dans la quantification de pré-gels de matrice extracellulaire hépatique.[3]
Produit utilisé comme substance de revêtement pour favoriser la croissance et la différenciation des cellules adhérentes.
Biochem/physiol Actions
Le collagène est essentiel à l'intégrité mécanique des tendons et des os. Le collagène issu de tendons de queue de rat est utilisé en ingénierie des tissus, particulièrement pour la génération de gels d'échafaudages tridimensionnels.[4] Il présente une faible antigénicité et est compatible avec les fibroblastes de gencives et les kératinocytes oraux humains.[5]
Features and Benefits
- Produit provenant de queues de rats.
- Stérilisé par filtration pour réduire au maximum les risques de contamination.
- Convient à un large éventail d'applications de culture cellulaire.
- Procure une couverture de surface optimale (6-10 μg/cm2) pour faciliter l'adhésion et la prolifération des cellules.
General description
Le collagène est une protéine de la matrice extracellulaire qui forme une structure en hélice triple. La structure du collagène de type I, en particulier, est caractérisée par la présence de trois chaînes α identiques sous sa forme homotrimérique. L'une de ces trois chaînes α présente une légère variation au niveau de sa séquence.[6] Le collagène de type I a initialement été isolé à partir de tendons de queue de rat et présente deux chaînes α principales (α1 et α2) et une chaîne β.[5]
Other Notes
La référence C3867 a un aspect gélifié lorsqu'on la retire pour la première fois de la glacière. Elle redevient liquide à 37 °C.
Le collagène comprend un certain nombre de types distincts du point de vue structurel et génétique. Nous utilisons la nomenclature proposée par Bornstein et Traub. Il est important de ne pas confondre la désignation des types utilisée par Sigma et les types de classification reconnus du collagène.
Preparation Note
Il est recommandé pour une utilisation comme substrat de culture cellulaire Il pourrait ne pas être adapté à la formation de gels tridimensionnels.
Solution stérile préparée à partir de tendons de queue de rat par une modification de la méthode d′extraction publiée 1. Ce produit est fourni sous forme de solution aqueuse dans de l′acide acétique à 20 mM et à une concentration indiquée sur le flacon (environ 100 mg de protéine par flacon). La concentration en protéine a été estimée par la méthode Biuret 2.
Une analyse par électrophorèse sur gel de polyacrylamide en présence de SDS présente un schéma typique de bandes pour le collagène de type I, à savoir un doublet de poids moléculaire apparent de 115 et 130 kDa et un autre doublet de 215 et 235 kDa. Selon les résultats de cette analyse, la pureté de l′échantillon de collagène est > 95 %.
Une analyse par électrophorèse sur gel de polyacrylamide en présence de SDS présente un schéma typique de bandes pour le collagène de type I, à savoir un doublet de poids moléculaire apparent de 115 et 130 kDa et un autre doublet de 215 et 235 kDa. Selon les résultats de cette analyse, la pureté de l′échantillon de collagène est > 95 %.
Related product
Réf. du produit
Description
Tarif
Classe de stockage
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Type I collagen extracted from rat-tail and bovine Achilles tendon for dental application: a comparative study
Techatanawat S, et al.
Asian Biomedicine : Research, Reviews and News, 5(6), 787-798 (2011)
Thermal helix-coil transition in UV irradiated collagen from rat tail tendon
Sionkowska A and Kaminska A
International Journal of Biological Macromolecules, 24(4), 337-340 (1999)
Preparation of ready-to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications
Rajan N, et al.
Nature Protocols, 1(6), 2753-2753 (2006)
Mechanisms of angiotensin II signaling on cytoskeleton of podocytes
Hsu HH, et al.
Journal of Molecular Medicine, 86(12), 1379-1394 (2008)
Ectopic adenine nucleotide translocase activity controls extracellular ADP levels and regulates the F1-ATPase-mediated HDL endocytosis pathway on hepatocytes
Cardouat G, et al.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1862(9), 832-841 (2017)
Articles
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
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Can rat collagen solution be used as coating for human cells or is it better to use human collagen solutions?
1 answer-
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Should I add collagen I to acetic acid to obtain 0.1% (w/v) collagen solution, or it's already added and I should only dilute it to working concentration which is 0.01?
1 answer-
Here are our preparation instructions:
This product is prepared from rat tail tendons by modification of a published procedure.
It is supplied as an aqueous solution in 20 mM acetic acid (~100 mg of protein per vial). Protein concentration was estimated by the biuret method.
The vial contains approximately 100 mg of protein. Volume per vial: 22–25 ml. The product ships on wet ice and storage at 2–8 °C is recommended. During shipment, or if the storage temperature for this product goes slightly below the recommended range, the product may acquire a gel-like appearance. Under such circumstances, it is possible to warm the product gently, to no more than 45 °C, in order to reconstitute the product fully, before use. Aliquots that have been diluted to a working concentration may be stored at 2–8 °C for up to 2 weeks. Do not freeze. Optimal conditions for attachment must be determined for each cell line and application.1. An appropriate volume of the collagen solution should be diluted to a working concentration of 0.01% using sterile, tissue culture grade water.
2. Coat dishes with 6-10 mg/cm2. Allow the protein to bind for several hours at room temperature or 37 °C, or overnight at 2–8 °C.
3. Remove excess fluid from the coated surface and allow it to dry overnight. If sterility has been compromised, the dried, coated surface can be sterilized easily by overnight exposure to UV light in a sterile tissue culture hood.
4. Rinse with sterile tissue culture grade water or a balanced salt solution before introducing cells and medium.Helpful?
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What will be the concentration of collagen? I am trying to download the CoA, but I am unable to download it.
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This product is prepared at a concentration of approximately 4 mg/mL. A package of 1 Vial will typically have a volume of approximately 35-40 mL. The exact concentration is lot specific and reported in the product Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/c3867#product-documentationHelpful?
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IVD conformity: Does this product have IVDD or IVDR conformity?
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This collagen is intended for research use only, and is not approved for IVD purposes.
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In what volume should I dilute this solution in order to make collagen-coated plates for tissue culture?
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The recommended surface coverage for this product is 6?10 μg/cm2. Collagen solutions may be diluted to a working concentration of 0.01%. A general collagen coating protocol is available at https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/collagen-product-protocols
The collagen concentration of this product is reported on a lot specific basis and will be required to prepare the working solution. See the link below to review a sample Certificate of analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/C3867-1VL0000255665.pdfHelpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can I make a 3-D collagen gel?
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Not all lots of C7661 are suitable for 3D gels. Product No. C4243 has been use-tested in this application. If C4243 is not available, we recommend that customers screen lots of C7661 to try to make 3D gels. We have found that collagen that has been sterilized by gamma-irradiation or by chloroform cannot be used to make 3D collagen gels. The following protocol can be used to make collagen gels: Materials required:Collagen gel solution 1.5 - 3 mg/mL 10X tissue culture medium containing phenol red Sodium bicarbonate or HEPES buffer Procedure: 1.Measure out 800 μL of collagen solution. 2.Add 100 μL of 10X medium (buffered with 1X sodium bicarbonate or HEPES) 3.Adjust pH with 1 N sodium hydroxide, if required (100 μl or less) 4.Add 10X medium to bring volume to 1 ml 5.Mix contents well. Solution should maintain red color to indicate physiological pH6. Dispense into wells to a depth of 1-2 mm (approx. 15 μL/well) 7.Transfer to 37 °C for 20-40 minutes 8.Examine for gel formation The above volumes represent quantities for use in 24 well plates. Volumes can be adjusted to accommodate any culture vessel.
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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How are collagen solutions stored?
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Collagen solutions should be stored in the refrigerator at 2-8 °C for up to 1 year, unless it becomes contaminated.
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Why am I seeing some insolubles in my collagen solution?
1 answer-
The solubility specifications for this product is clear to hazy colorless solution with a few insolubles at 1 mg/ml in water with 2 μl acetic acid (or 0.1 N acetic acid). The insolubles can be removed by settling or centrifugation.
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