The solution storage and stability of this product have not been established. However, the activated or nicked version of this material, product D4522, notes that Solutions of DNA have been stored successfully for several months at 4 °C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. Please see the link below to review additional information available in the datasheet for the D4522:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/630/619/d4522pis.pdf
D4764
Deoxyribonucleic acid from calf thymus
Genomic, unsheared
Synonyme(s) :
double-stranded template DNA, Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
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0.25 UNIT
48.90 CHF
1 UNIT
124.00 CHF
5 UNITS
392.00 CHF
48.90 CHF
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A propos de cet article
Numéro CAS:
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
293-507-3
NACRES:
NA.52
MDL number:
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Laissez-nous vous aiderNom du produit
Deoxyribonucleic acid from calf thymus, Genomic, unsheared
biological source
bovine (calf) thymus
grade
Molecular Biology
form
lyophilized powder
mol wt
>20 kb
storage temp.
2-8°C
Quality Level
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1 of 4
Cet article | D8899 | D4522 | D8515 |
|---|---|---|---|
| grade for molecular biology | grade for molecular biology | grade for molecular biology | grade - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt >20 kb | mol wt ~50 kb | mol wt - | mol wt - |
Application
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay.[1] It was used as reference to determine the DNA content in cartilage tissue digest samples.[2][3]
Biochem/physiol Actions
42% GC; Tm = 87 °C.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
Features and Benefits
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
• High quality template DNA
General description
High quality double-stranded template DNA isolated from the thymus of male and female calves.
Preparation Note
Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A260 units per mL. ~20 A260 units per mg DNA.
comparable product
Réf. du produit
Description
Tarif
related product
Classe de stockage
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Roel J H Custers et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 28(4), 429-435 (2009-11-04)
The purposes of the present study were to explore the surgical possibilities for replacement of the medial tibial plateau by a metallic implant in a large animal model and to examine the implications for the opposing cartilage. In six goats
A Châtel et al.
Ecotoxicology (London, England), 20(8), 1727-1740 (2011-06-10)
In the present study, we analyzed the effects of two major pollutants of the environment, tributyltin (TBT) and water-accommodated fraction (WAF) of diesel oil, on MAP kinase activation, apoptosis induction and DNA damage, in the marine sponge Suberites domuncula. Our
Nathalie W D Jansen et al.
Arthritis and rheumatism, 56(1), 199-207 (2006-12-30)
Joint bleeding, or hemarthrosis, leads in time to severe joint damage. This study was carried out to test the in vitro thresholds of exposure time and concentration that lead to irreversible joint damage, to add to the discussion on the
Sutha Shobana et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 107, 117-132 (2013-02-19)
Equilibrium studies of Ni(II), Cu(II) and Zn(II) mixed ligand complexes involving a primary ligand 5-fluorouracil (5-FU; A) and imidazoles viz., imidazole (him), benzimidazole (bim), histamine (hist) and L-histidine (his) as co-ligands(B) were carried out pH-metrically in aqueous medium at 310±0.1K
K Bishayee et al.
Cell proliferation, 46(2), 153-163 (2013-03-21)
Small aromatic compounds like flavonoids can intercalate with DNA molecules bringing about conformational changes leading to reduced replication and transcription. Here, we have examined one dietary flavonoid, quercetin (found in many fruit and vegetables), for possible anti-cancer effects, on HeLa
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Datasheet
Instructions
Datasheet
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What are the storage conditions once the DNA is resuspended? Should I keep the DNA at -20ºC or -80ºC?
1 answer-
Helpful?
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how much is 1 unit (i.e. in ug or mg)?
1 answer-
One unit, based on the A260 value, is approximately 50 µg. The general conversion formula is 20 units per milligram. The 0.25 UN package size will contain approximately 12.5 µg, the 1 jUN package size will contain approximately 50 µg, and the 5UN package size will contain approximately 250 µg.
For additional product information, please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/289/542/d4764dat.pdfHelpful?
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