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Merck

IP05

Protein G Plus/Protein A Agarose Suspension

Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Sinonimo/i:

Affinity resin, Protein G/Agarose

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Informazioni su questo articolo

Codice UNSPSC:
41116133
NACRES:
NA.56

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Stato

slurry (Liquid)

contiene

≤0.1% sodium azide as preservative

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

do not freeze

tecniche

protein purification: suitable

Compatibilità

suitable for microbiology

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Categorie correlate

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technique(s)

protein purification: suitable

technique(s)

immunoprecipitation (IP): suitable

technique(s)

-

technique(s)

protein purification: suitable

form

slurry (Liquid)

form

slurry (Liquid)

form

slurry (Liquid)

form

slurry (Liquid)

suitability

suitable for microbiology

suitability

suitable for immunoprecipitation

suitability

-

suitability

suitable for microbiology

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

contains

≤0.1% sodium azide as preservative

contains

≤0.1% sodium azide as preservative

contains

≤0.1% sodium azide as preservative

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

Descrizione generale

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Applicazioni

Immunoprecipitation (see comments)

Stato fisico

33% slurry in PBS.

Altre note

Agarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Toxicity: Standard Handling (A)

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Contenuto correlato

We manufacture IP and antibody purification agarose beads that purify immunoglobulins and IgG fractions. Our kits also provide a total antibody purification process

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