04529

Nitrilase

recombinant, expressed in E. coli, ≥2.0 U/mg

• Sinonimo/i: Nitrile aminohydrolase
• Numero CAS: 9024-90-2
• UNSPSC Code: 12352204
• NACRES: NA.54
• Numero CE: 3.5.5.1 (BRENDA, IUBMB)
• MDL number: MFCD04040969
• Specific activity: ≥2.0 U/mg
• Recombinant: expressed in E. coli

Proprietà

• recombinant: expressed in E. coli
• form: crystals, powder
• specific activity: ≥2.0 U/mg
• mol wt: 41 kDa
• storage temp.: 2-8°C

Descrizione

General description

Nitrilase belongs to the C-N hydrolase superfamily. It possesses the catalytic triad motifs: glutamate, lysine, and cysteine and are distributed in fungi, plants, and animals.The isoelectric point (pI) of this protein is 8.1. Native polyacrylamide gel electrophoresis (PAGE).

Application

Nitrilase may be used for immobilization on acryloyl crosslinked cellulose dialdehyde (ACCD) for catalytic activity studies.

Biochem/physiol Actions

Nitrilases catalyze the liberation of ammonia and the formation of carboxylic acid from the nitriles. The oligomeric structural organization of nitrilase is crucial for its functionality. It displays broad substrate specificity.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

1 U corresponds to the amount of enzyme which liberates 1 μmol ammonia per minute at pH 7.2 and 25°C with the conversion of acrylonitrile to acrylic acid

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Parola di avvertenza: Danger
• Codici di pericolo: H317,H334
• Avvertenze di sicurezza: P261 - P280 - P342 + P311
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Classe di stoccaggio: 11 - Combustible Solids
• WGK: WGK 3
• Punto di infiammabilità (°F): Not applicable
• Punto di infiammabilità (°C): Not applicable