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76216

Papain from Carica papaya

solution, light brown, ≥10 U/mg protein (~25 mg/ml)

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Voi/SKUDisponibilitàPrezzo
50 mg
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CHF 183.00
250 mg
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CHF 307.00
1 g
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CHF 837.00

Informazioni su questo articolo

Numero CAS:
UNSPSC Code:
12352204
eCl@ss:
42030311
EC Number:
232-627-2
NACRES:
NA.54
MDL number:
Numero CE:
Specific activity:
≥10 U/mg protein (~25 mg/ml)
Biological source:
papaya

CHF 183.00


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biological source

papaya

Quality Segment

form

solution

specific activity

≥10 U/mg protein (~25 mg/ml)

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

color

light brown

storage temp.

2-8°C

General description

Papain is a proteolytic enzyme and is naturally occurring in papaya (Carica papaya L.). It is obtained from the latex of cut unripe papaya. Papain belongs to the cysteine proteinase family. It is a globular, single-chain protein composed of four disulfide bridges and catalytically essential residues. Papain consists of an all α-domain and anti-parallel β-sheet domain.

Application

Papain from Carica papaya has been used:

  • in the enzymatic dissociation of rat cortices to extract astrocytes for cell culture
  • to study its effects on Alpaca seminal plasma viscosity and sperm function
  • to study its effects on the yield and cryo-survival of Rhinoceros spermatozoa
Papain is used as a meat tenderizer. It′s also used to break up tissue in the preparation of primary cell cultures.

Biochem/physiol Actions

Papain is a cysteine protease that cleaves peptide bonds and is known to cleave the Fc portion of antibodies.
Papain plays an important role in many biological processes in many living organisms. It exhibits proteolytic activity against proteins, amino-acid esters and amide links, and short-chain peptides. Papain preferentially cleaves the peptide bonds of basic amino acids such as arginine, phenylalanine, and lysine. It has a wide range of applications in drug discovery, pharmaceutical, food, baking, and dairy industries.

Other Notes

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-benzoyl-L-arginine ethyl ester (BAEE, Cat. No. 12880) per minute at pH 6.2 and 25°C

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Questo articolo
P5306P31251495005
USP

USP

1495005

Papain

description

solution, light brown, ≥10 U/mg protein (~25 mg/ml)

description

lyophilized powder, aseptically filled

description

buffered aqueous suspension, 2× Crystallized, ≥16 units/mg protein

description

United States Pharmacopeia (USP) Reference Standard

technique(s)

single cell analysis: suitable, cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

biological source

papaya

biological source

papaya (latex)

biological source

-

biological source

-

specific activity

≥10 U/mg protein (~25 mg/ml)

specific activity

≥8.0 units/mg protein

specific activity

≥16 units/mg protein

specific activity

-

form

solution

form

lyophilized powder

form

buffered aqueous suspension

form

solid

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

-


Pittogrammi

Health hazardExclamation mark

Parola di avvertenza

Danger

Codici di pericolo

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Classe di stoccaggio

11 - Combustible Solids

Che succede?

WGK 1

Punto di infiammabilità (°F)

Not applicable

Punto di infiammabilità (°C)

Not applicable

DPI (dispositivi di protezione individuale)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



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Questions

  1. Which solution is used in Papain from Carica papaya solution? DMSO? water?

    1 answer
    1. This product is an aqueous solution containing 35 - 55% glycerol.

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