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A3151

Avidin–Peroxidase

lyophilized powder

Sinonimo/i:

Avidin-POD

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Voi/SKUDisponibilitàPrezzo
1 mg
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CHF 130.00
2 mg
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CHF 220.00
5 mg
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CHF 490.00

Informazioni su questo articolo

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00130587
Form:
lyophilized powder
Biological source:
avidin from egg white, enzyme from horseradish

CHF 130.00

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Quality Segment

biological source

avidin from egg white, enzyme from horseradish

conjugate

peroxidase conjugate

form

lyophilized powder

composition

Protein, ≥70% E1%/280

extent of labeling

0.7-2.0 mol peroxidase per mol avidin

technique(s)

direct ELISA: 1:50,000

solubility

H2O: soluble 1 mg/mL, PBS: soluble

storage temp.

−20°C

General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin-Peroxidase has been used for detecting anti-5-bromodeoxyuridine (anti-BrdU) binding and OVA-IgG1.
To detect Ga1-deficient IgA1 antibodies in mouse serum, ELISA assays were performed using sera which was incubated with biotinylated HAA, which binds IgA1, in 96-well plates. HRP-conjugated avidin was then incubated with the sera for 40 minutes at 37 degrees.

Physical form

Lyophilized powder containing citrate buffer.

Preparation Note

Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Questo articolo
A7419A7294A9275
Avidin–Peroxidase lyophilized powder

Sigma-Aldrich

A3151

Avidin–Peroxidase

Avidin–Peroxidase buffered aqueous solution

Sigma-Aldrich

A7419

Avidin–Peroxidase

Avidin–Alkaline Phosphatase buffered aqueous solution

Sigma-Aldrich

A7294

Avidin–Alkaline Phosphatase

Avidin from egg white BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

Sigma-Aldrich

A9275

Avidin from egg white

description

lyophilized powder

description

buffered aqueous solution

description

buffered aqueous solution

description

BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

biological source

enzyme from horseradish, avidin from egg white

biological source

enzyme from horseradish, avidin from egg white

biological source

enzyme from bovine (calf) intestine, avidin from egg white

biological source

egg white

technique(s)

direct ELISA: 1:50,000

technique(s)

indirect ELISA: 1:100,000, western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well)

technique(s)

western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane, direct ELISA: 1:70,000

technique(s)

-

form

lyophilized powder

form

buffered aqueous solution

form

buffered aqueous solution

form

lyophilized powder

solubility

PBS: soluble, H2O: soluble 1 mg/mL

solubility

-

solubility

-

solubility

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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Pittogrammi

Health hazard
GHS08

Parola di avvertenza

Danger

Codici di pericolo

H334

Avvertenze di sicurezza

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Classe di stoccaggio

11 - Combustible Solids

Che succede?

WGK 1

Punto di infiammabilità (°F)

Not applicable

Punto di infiammabilità (°C)

Not applicable

DPI (dispositivi di protezione individuale)

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number

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