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A3151

Avidin–Peroxidase

Name: Avidin–Peroxidase
Brand: SIGMA
Price: 125 CHF

lyophilized powder

Sinonimo/i:

Avidin-POD

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Voi/SKU	Disponibilità	Prezzo
1 mg	Per conoscere la disponibilità, visualizza il carrello	CHF 125.00
2 mg	Per conoscere la disponibilità, visualizza il carrello	CHF 212.00
5 mg	Per conoscere la disponibilità, visualizza il carrello	CHF 473.00

Informazioni su questo articolo

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00130587

Form:

lyophilized powder

Biological source:

avidin from egg white, enzyme from horseradish

CHF 125.00

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Proprietà

Quality Segment

200

biological source

avidin from egg white, enzyme from horseradish

conjugate

peroxidase conjugate

form

lyophilized powder

composition

Protein, ≥70% E1%/280

extent of labeling

0.7-2.0 mol peroxidase per mol avidin

technique(s)

direct ELISA: 1:50,000

solubility

H₂O: soluble 1 mg/mL, PBS: soluble

storage temp.

−20°C

Descrizione

General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin-Peroxidase has been used for detecting anti-5-bromodeoxyuridine (anti-BrdU) binding and OVA-IgG₁.

To detect Ga1-deficient IgA1 antibodies in mouse serum, ELISA assays were performed using sera which was incubated with biotinylated HAA, which binds IgA1, in 96-well plates. HRP-conjugated avidin was then incubated with the sera for 40 minutes at 37 degrees.

Physical form

Lyophilized powder containing citrate buffer.

Preparation Note

Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Questo articolo	A7294	A2901	A9207
Sigma-Aldrich A3151 Avidin–Peroxidase	Sigma-Aldrich A7294 Avidin–Alkaline Phosphatase	Sigma-Aldrich A2901 Avidin–FITC from egg white	Sigma-Aldrich A9207 Avidin−Agarose from egg white
description lyophilized powder	description buffered aqueous solution	description lyophilized powder	description aqueous glycerol suspension
biological source enzyme from horseradish, avidin from egg white	biological source enzyme from bovine (calf) intestine, avidin from egg white	biological source -	biological source egg white
technique(s) direct ELISA: 1:50,000	technique(s) western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane, direct ELISA: 1:70,000	technique(s) immunohistochemistry: suitable	technique(s) -
form lyophilized powder	form buffered aqueous solution	form lyophilized powder	form aqueous glycerol suspension
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C
solubility H₂O: soluble 1 mg/mL, PBS: soluble	solubility -	solubility -	solubility -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200