Passa al contenuto
Merck

Documenti fondamentali

Visualizza tutta la documentazione

Vai a

A7653

L-Alanine Dehydrogenase from Bacillus subtilis

buffered aqueous glycerol solution, ~30 units/mg protein (Lowry)

Sinonimo/i:

L-Alanine: NAD+ oxidoreductase (deaminating)

Autenticati per visualizzare i prezzi organizzativi e contrattuali.

Scegli un formato

Cambia visualizzazione
Voi/SKUDisponibilitàPrezzo
100 units
Per conoscere la disponibilità, visualizza il carrello
CHF 352.00

Informazioni su questo articolo

Numero CAS:
9029-06-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-847-9
MDL number:
MFCD00131441
Numero CE:
1.4.1.1 (BRENDA, IUBMB)
eCl@ss:
32160701
Specific activity:
~30 units/mg protein (Lowry)
Biological source:
Bacillus subtilis

CHF 352.00

Per conoscere la disponibilità, visualizza il carrello
​
Servizio Tecnico
Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.
Permettici di aiutarti

biological source

Bacillus subtilis

Quality Segment

form

buffered aqueous glycerol solution

specific activity

~30 units/mg protein (Lowry)

foreign activity

LDH ~1% (using pyruvate as substrate)

storage temp.

−20°C

Application

L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection .

Biochem/physiol Actions

L-Alanine dehydrogenase is an A-stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate, and NADH are released in that specific order. Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.

Physical form

Solution in 50% glycerol containing 10 mM potassium phosphate buffer, pH 7.7

Other Notes

One unit will convert 1.0 μmole of L-alanine to pyruvate and NH3 per min at pH 10.0 at 25 °C.

Confronta articoli simili

Visualizza il confronto per intero

Mostra le differenze

1 of 1

Questo articolo
A7189L5135431525
L-Alanine Dehydrogenase from Bacillus subtilis buffered aqueous glycerol solution, ~30 units/mg protein (Lowry)

Sigma-Aldrich

A7653

L-Alanine Dehydrogenase from Bacillus subtilis

L-Alanine Dehydrogenase from Bacillus subtilis ammonium sulfate suspension, ≥20 units/mg protein (Lowry)

Sigma-Aldrich

A7189

L-Alanine Dehydrogenase from Bacillus subtilis

L-Leucine Dehydrogenase from Bacillus cereus lyophilized powder, ≥60 units/mg protein

Sigma-Aldrich

L5135

L-Leucine Dehydrogenase from Bacillus cereus

L-Leucine Dehydrogenase, Bacillus cereus

Sigma-Aldrich

431525

L-Leucine Dehydrogenase, Bacillus cereus

description

buffered aqueous glycerol solution, ~30 units/mg protein (Lowry)

description

ammonium sulfate suspension, ≥20 units/mg protein (Lowry)

description

lyophilized powder, ≥60 units/mg protein

description

-

specific activity

~30 units/mg protein (Lowry)

specific activity

≥20 units/mg protein (Lowry)

specific activity

≥60 units/mg protein

specific activity

≥50 units/mL, ≥10 units/mg protein

biological source

Bacillus subtilis

biological source

Bacillus subtilis

biological source

-

biological source

Bacillus sp. (Bacillus cereus)

form

buffered aqueous glycerol solution

form

ammonium sulfate suspension

form

lyophilized powder

form

liquid

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

foreign activity

LDH ~1% (using pyruvate as substrate)

foreign activity

-

foreign activity

-

foreign activity

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

Still not finding the right product?

Explore all of our products under L-Alanine Dehydrogenase from Bacillus subtilis

Pittogrammi

Health hazard
GHS08

Parola di avvertenza

Danger

Codici di pericolo

H334

Avvertenze di sicurezza

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Classe di stoccaggio

10 - Combustible liquids

DPI (dispositivi di protezione individuale)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documenti section.

Se ti serve aiuto, non esitare a contattarci Servizio Clienti

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Questions

Reviews

No rating value

Active Filters